常用健脾益氣中藥紅耆對胰臟胰島素分泌作用之探討

The Study of Hong-Qi on Insulin Secretion in Perfused Rat Pancreas

本研究目的在探討台灣民眾常用健脾益氣中藥紅耆對胰臟胰島素分泌之作用。研究選用四組不同劑量（650mg、390mg、130mg、control）之紅耆粗萃取物灌流大鼠胰臟，再以競爭性放射性免疫法（radioimmunoassay，RIA）來測定胰島素濃度之變化，研究結果以ANOVA分析在不同劑量與時間作用下胰島素之分泌情形。結果發現使用紅耆灌流大鼠胰臟，在2-4分鐘的內即可刺激胰島素分泌，並可達最大分泌高峰，亦同時存在「劑量-效應」（dose-dependent）關係。紅耆刺激胰島素分泌達到高峰後，即隨著時間以緩降趨勢下降。而胰島細胞經藥物灌流後，對葡萄糖的刺激仍有反應，表示胰島細胞未遭破壞。結論（1）以紅耆灌流大鼠胰臟刺激胰島素分泌，有立即顯著效果。（2）紅耆可刺激β細胞分泌胰島素，但是無法發動β細胞之生化合成機制。（3）在0.5c.c.（650mg）濃度以下，胰島素之分泌，隨紅耆劑量升高而分泌增加。

Hong-Qi is one of the most popular Spleen-Fostering and Qi-Supple-menting Chinese herbs used in Taiwan. The purpose of this study was to evaluate the effects of Hong-Qi on insulin secretion in perfused rat pancreas. Four doses (650 mg, 390 mg, 130 mg, control) of Hong-Qi crew extract were used. The perfused rat pancreas was adopted with competitive radioimmunoassay to evaluate the change of insulin secretion induced by Hong-Qi. The statistical analysis was performed using ANOVA. Our result showed the perfusion of Hong-Qi could stimulate the secretion of insulin, and obtain the obvious peak in dose-dependent response. Once the peak was obtained, the secretion of insulin decreased gradually. The insulin secretion function of islet cells remained intact after perfusion.