覆盆莓對大鼠體重和血脂的影響

Effects of Raspberry on the Body Weight and Blood Lipids of Rats

為了探討覆盆莓對體重和脂肪代謝的影響，本實驗將30隻5週大SD雄性大鼠以商業配方飼養一週後，隨機分為3組， 分別為對照組(C)、對照組飲食加5%覆盆莓組(R5)、對照組飲食加10%覆盆莓(R10)，於每週測量老鼠體重並每隔二天測量老鼠攝食量。六週後以斷頸法犧牲所有老鼠，取動物血液、肝臟及脂肪組織進行分析。結果顯示第四週時各組大鼠體重有顯著差異，攝食覆盆莓的動物體重低於未攝食覆盆莓者。攝取覆盆莓的動物的肝臟及脂肪組織重量低於未攝取覆盆莓的動物。添加覆盆莓會降低大鼠三酸甘油醋，但只有10%覆盆莓會降低總膽固醇和低密度脂蛋白膽固醇。以西方點墨法分析脂質代謝相關蛋白質的表現，結果顯示攝取覆盆莓的大鼠的肝臟乙醯──輔酶A：膽固醇乙醯轉移酯(acyl-CoA:cholesterol acyltransferase)和原漿微粒三酸甘油酯轉移蛋白(microsomal triglyceride transfer protein)的表現有減少的趨勢、HMG-CoA還原酶(HMG-CoA reductase)的表現有增加的趨勢，但這些結果並未有統計上的顯著性。

To study the effect of raspberry on the body weight and lipid metabolism, thirty 5-week old SD male rats were assigned to one of the three groups after maintained on the chow diet for one week: control diet (C), control diet supplemented with 5% raspberry (R5), and control diet supplemented with 10% raspberry (R10). The body weight and diet intake were measured once a week and every other day, respectively. After keeping in the diets for six weeks, all animals were sacrificed to collect the blood, liver, and adipose tissues for further analysis. The results indicated that animals supplemented with raspberry started to show lower body weight compared with animals in regular group after maintaining in the diets for 4 weeks. The rats supplemented with raspberry also had lower liver and adipose tissue weight. Supplementation of raspberry decreased blood triglyceride level, but only 10% raspberry supplementation showed lowering effect on total cholesterol and low density lipoprotein-cholesterol (LDL-C). The animals in R5 and R10 groups had lower liver and adipose tissue weights compared with those in regular group. Western blot analysis indicated that supplementation of raspberry down-regulated the expression of acyl-CoA:eholesterol acyltransferase (ACAT) and microsomal triglyceride transfer protein (MTP), and up-regulated the expression of HMG-CoA reductase; however, the results had no statistical significance.