僵直性脊椎炎病患及其第一等親在周邊血T細胞受器的Vß基因表現

Peripheral blood T cell receptor Vβ gene expressions in patients with ankylosing spondylitis and their first-degree relatives

本文主要藉由僵直性脊椎炎病患及其第一等親有HLA-B27和無HLA-B27者，其Vß基因的表現，探討與僵直性脊椎炎的相關性。在24個僵直性脊椎炎家族中，將病患分為僵直性脊椎炎（n=36）、僵直性脊椎炎病患第一等親HLA-B27陽性（n=24）及僵直性脊椎炎病患第一等親HLA-B27陰性（n=24）三組，測其周邊血T細胞受器的Vß基因表現與其貝氏僵直性脊椎炎疾病活動評量表。結果發現T細胞受器Vß14的表現在僵直性脊椎炎病患和第一等親HLA-B27陽性者比第一等親HLA-B27陰性者更明顯（p<0.05），顯示其很可能是與HLA-B27分子相接觸的位置。另外，T細胞受器Vß13.6和僵直性脊椎炎的疾病活動度可能有明顯相關（p<0.05）。

Objective. To investigate T cell Vβ genes expression in patients with ankylosing spondylitis (AS), and whether there are any different expressions of peripheral blood T cell receptor (TCR) vβ in AS patients and their first-degree relatives (FDR). Methods. Twenty-four families with thirty-six HLA-B27+ AS patients and their 48 FDR were divided into three groups: HLA-B27+ AS patients (n=36), their FDR who had B27 positive (B27+, n=24) and their FDR who had B27 negative (B27-, n=24). The peripheral blood T cell receptor vβ gene was investigated using 10 different vβ antibodies and the flowcytometric method. Results. The data revealed a significantly higher expression of TCR VΒ14 in B27+ AS patients and their B27+ FDR than in B27- FDR (p<0.05). Moreover, CD8+ T cells had significantly increased expressions of peripheral TCR Vl33, Vf39, VBJ4, and Vl320 in AS than in their B27- FDR (p= 0.01, 0.03, 0.01, 0.03 respectively). Moderate/severe disease activity had a higher expression of TCR Vβ13.6 compared to mild disease activity in AS patients. Conclusion. These results imply that TCR vβ 14 chain usage was a preferred site for contact with the HLA-B27 molecule. AS patients bad CD8+ T cells autoreactivity to a HLA-B27-restricted self-epitope. TCR vβ 13.6 may be associated with the disease activity of AS.