以化學光反應測定人類顆粒性白血球與單納尿酸晶體間之反應

Lucigenin-Dependent Chemiluminescence Produced by the Interaction Between Human Neutrophils and Monosodium Urate Crystals

本研究利用吞噬反應時釋出化學光的特性，研究多核形白血球與單鈉尿酸結晶 體之間的發炎反應。實驗進行前單鈉尿酸結晶體與各類蛋白質先行培養以證明結晶體須接受調理作用方能使白血球誘發炎症反應。在各類調理素中，人類正常全血清的調理作用最強，化學光反應達15.6 ± 2.0mv，但經去補體後，此數值劇跌至只剩6.4 ± 0.6mv，再經少量天竺鼠補體蛋白部份補足後，化學光反應又可回復至原來水準(16.4 ± 1.9mv)。天竺鼠補體本身並不能產生夠大的調理作用（化學光反應值只有3.3 ± 0.9mv)，但對其他調理素的功能卻有加成的提高作用。IgG的中低度調理能力經補體加入後可從原來的4.7 ± 0.4mv增加3倍之多(13.3 ± 1.8mv)。IgA與IgM的調理作用很弱，也不能經由補體加以提升。人類血清蛋白質無法吸附在晶體表面上，沒有調理作用的能力。綜觀本研究結果，IgG與補體蛋白的緊密合作可能是刺激白血球引發痛風關節炎急性發作的原因之一。

The interaction between normal human granulocytes and microcrystal- line monosodium urate monohydrate (MSUC) was studied by an assay method of lucigenin-dependent chemiluminescence. Various proteins were adsorbed onto the crystals by incubation in order to clarify the requirement of opsonization for MSUC to initiate an inflammatory reaction. Among them, normal human serum (NHS) displayed the highest opsonic activity with tbe chemiluminescence of 15.6 ± 2.0 mV which was, however, markedly diminished to 6.4 ± 0.6 mV if it was decomplemented. Nevertheless, partial reconstitution of the decomplemented NHS with a samll amount of guinea pig complement, whose opsonic activity was quite low (33 ± 0.9 mV), could restore the reaction to 16.4 ± 1.9 mV. This synergetic effect on the opsonic activity was also seen in the interaction between PMNs and IgG-adsorbed crystals which was originally only moderately high (4.7 ± 0.4 mV), but showed 3 times augmentation of chemiluminescence after the guinea pig complement proteins were added. IgA and IgM were weak in opsonic activity and not influenced by complement. Human albumin was unable to adsorded onto the crystal surface. It was therefore possible that cooperation between IgG and complement proteins were essential for PMNs to Initiate an acute episode of gouty arthritis.