胎盤幹細胞分化為神經細胞之蛋白質體分析

Comparative proteomic analysis of Placenta-Derived Multipotent Cells (PDMCs) and its differentiated Neuron

胎盤幹細胞定義為一群來自胎盤之具有分化及自我增生能力之細胞。蛋白質體學則為一種以高過量研究生物內蛋白質體之研究方法，蛋白質體研究方法主要為二維膠體電泳搭配上質譜儀及生物資訊學分析，二維膠體電泳主要之功能在將蛋白質體內之蛋白質分離，並加以定量。而質譜儀及生物資訊學則在處理蛋白質身份鑑定以及比對生物資料庫。我們運用IBMX這種藥物處理胎盤幹細胞可誘導其分化為神經細胞，我們利用蛋白質體學之分析方法嘗試去研究達其中的蛋白質體變化，我們比較了許多受此藥物影響之蛋白質表現量，想從其中發現真正調控胎盤幹細胞之關鍵生物分子。透過此研究將會讓我們暸解胎盤幹細胞分化為神經細胞之生理機縛，並在將來提供臨床疾病治療之新方向。

Placenta-derived multipotent cells (PDMCs) are defined as a group of cells with differentiating and self-renewal properties. The proteomic experimental approach is analyzing a proteome in a high-through-put way. The field of proteomics has several technologies including two-dimensional gel eletrophoresis (2-DE) for protein separation and quantification, mass spectrometry-based protein identification and bioinformatics that integrated technologies. We used IBMX to induce the differentiation of PDMCs to neuron. Further, those cells would serve as a model to apply proteomics strategy to global profile the proteome of PDMCs and its differentiated neuron. We also compared the up- and down-regulated proteins during PDMCs differentiation trying to find the key bio-factors behind PDMCs differentiated into neuron. We believe those findings will give as several candidates for key players in the neuron differentiation. It will help us decipher the mechanism of neuron differentiation and provide us a new version of disease treatment.