Systematic Analysis of Antibiotics via Agar Gel Electrophoresis and Antimicrobial Spectrum Candidacy for Detecting Residual Antibiotics in Foods

Systematic analysis of antibiotics via agar gel electrophoresis and antimicrobial spectrum, a method previously developed in our laboratory, was modified to increase its resolution power and sensitivity. Six test organisms were employed in the analysis system so that antibiotics of different chemical groups can be distinguished from each other. Optimal conditions of the height of agar gel and test organism strip, and the width of sample slit were determined and modifications were made which enable the analysis of antibiotics at the concentration levels of food residues. Other single procedure and reproducibility were together discussed so as to evaluate its candidacy for detecting residual antibiotics in food.