歸脾湯對STZ誘發糖尿病鼠學習記憶障礙之研究

The Study of Effects of Gui-Pi-Tang on STZ-induced Diabetic Memory Dysfunction Rats

本研究探討歸脾湯（GPT）（1g/kg）對streptozotocin（STZ）誘發高血糖鼠在水迷宮學習障礙之影響，進而初步了解GPT預防或治療糖尿病暨學習障礙之療效，提供中醫臨床用藥參考。將老鼠隨機分為四組：（一）空白對照組，（二）STZ誘導糖尿病組，（三）GPT預防組，（四）GPT治療組。首先進行GPT對STZ誘發高血糖鼠葡萄糖耐受性實驗，結果顯示，GPT預防組對於口服葡萄糖後，血糖及血中insulin濃度變化與STZ誘發組比較，並無顯著差異；而GPT治療組對於口服葡萄糖後血糖變化比STZ誘發組低（p＜0.001）；而血中insulin濃度並無差異。其次，針對GPT對STZ誘發高血糖鼠學習記憶之研究，結果顯示，GPT治療組顯著改善STZ誘發高血糖鼠空間性學習操作障礙（p＜0.05）；GPT不論治療組或預防組均可改善STZ誘發高血糖鼠參考記憶障礙之現象（p＜0.05,p＜0.01）；預防組可改善STZ誘發高血糖鼠工作記憶再學習障礙之現象（p＜0.01）；而治療組可改善STZ誘發高血糖鼠工作記憶再現障礙之現象（p＜0.01）。綜上述結果可知GPT對STZ誘發高血糖鼠之學習記憶障礙有改善作用，且可改善STZ誘發高血糖鼠在水迷宮學習操作、參考記憶及工作記憶之障礙，其作用機制可能與改善血液葡萄糖之調節能力有關。

The purpose of this study was to investigate the preventive and therapeutic effect of Gui-Pi-Tang (GPT) on the impairment of water maze performance and hyperglycemia induced by streptozotocin (STZ). We measured oral glucose tolerance and behavior in order to understand the mechanism of the preventive and attenuating effects of GPT, and then provided TCM clinical drug reference. We grouped rats into four categories: the first group: treated with vehicle, the second group: induced by STZ, the third group: STZ-induced diabetic rats pretreated with GPT, the forth group: STZ-induced diabetic rats treated with GPT. The results show, GPT prevention group for oral glucose, blood sugar and blood insulin concentration changes compared with the STZ-induced group, no significant differences; and GPT treatment group for changes in blood glucose after oral glucose lower than the STZ-induced group; and there is no difference in blood insulin concentrations. Secondly, GPT on STZ-induced hyperglycemia study of learning and memory in rats, GPT treatment group significantly improve STZ-induced hyperglycemia in rat spatial learning (p ＜0.05); GPT treatment or prevention group can improve STZ-induced hyperglycemia rats reference of memory impairment (p ＜0.05, p ＜0.01); prevention group STZ-induced hyperglycemia can improve working memory in rats (p ＜0.01); and treated STZinduced hyperglycemia can improve working memory in rats (p ＜0.01). From these above results, we found that that GPT given before STZ treatment attenuated STZ-induced diabetic and the deficits in water maze performance, reference memory and working memory. We further suggest that therapeutic effects of GPT in STZ-induced diabetic rats might be due to improving glucose regulation.