肉類中磺胺劑殘留量檢測之探討

DETERMINATION AND INVESTIGATION OF SULFADRUG RESIDUES IN MEAT

本試驗之目的在探討行政院衛生署公告「食品中動物用禁殘留量檢驗方法－－磺胺劑之檢驗」及臺灣區肉品發展基金會現行採用之「肉品中磺胺劑殘留分析－－快速初檢方法（稀硫酸法）」，以薄層層析定性方法比較其差異性，並依動物用藥殘留標準0.1 ppm分別添加標準品做回收試驗，再以HPLC法測其回收率，結果兩種方法平均回收率分別為80.2%，59.9%。於八十三年十一月間在高雄市傳統市場隨機價購70件溫體豬肉檢體，依上述二種薄層層析法進行磺胺劑之檢驗，結果有三件檢體在兩種不同方法顯示磺胺二甲嘧啶（Sulfamethazine）均為陽性反應。依公告方法以HPLC定量結果其含量介於0.46ppm至0.70ppm之間，其餘檢體均未檢出。

The purpose of this experiment is to compare the 'Investigation method for residual amount of medicine in food' announced by Department of Health, Executive Yuan, and 'Fast screen test in analysis of residual sulfadrug in meat'. First we compared their differences by using 2 thin layer chromatorgraphy methods, then recovery experiments according to standards for residual medicine in animals, 0.1 ppm, and measurements for the rate of recovery using HPLC method were used. The results show that the rates of recovery for the two methods are 80.2%, 59.9%, respecitively. 70 meat samples purchased randomly from Kaohsiung markets in Nov .1994 were analyzed. According to the two thin layer chromatography methods mentioned above. Three of the samples showed pos itive In Sulfamethazine residue analysis by both methods with amounts ranging between 0.46 ppm and 0.70 ppm as measured by HPLC. And the others showed negative results for both in vestigation methods.