應用GC, HPLC 同時分離定性Heroin HCl, Morphine HCl, Pentazocine, Cocaine HCl, Caffeine, Diphenhydramine HCl, Procaine HCI Chlorpheniramine Maleate

SIMULTANEOUS SEPARATION AND IDENTIFICATION OF HEROIN HCI, MORPHINE HCI, PENTAZOCINE, CAFFEINE DIPHENHYDRAMINE HCI, PROCAINE HCI, CHLORPHENIRAMINE MALEATE BY GC AND HPLC

本研究條利用氣相層析法及液相層析法同時分離定性鹽酸海洛因 （Heroin HCl）、鹽酸嗎啡（Morphine HCl），潘他唑新（Pentazocine 俗稱速賜康，孫悟空），鹽酸古柯生僉（Cocaine HCl）及海洛因中常被個別摻加之藥物如鹽酸普魯卡因（Procaine HCl），咖啡生僉（Caffeine）鹽酸二苯胺明（Diphenhydramine HCl）以及潘他唑新中常被摻加之蘋果酸氯菲胺明（Chlorpheniramine Maleate）或 Diphenhydramine HCl 等；結果顯示為一簡單迅速之系統分析法。氣相層析條件為：玻璃層析管61 X 1/4' X 2mm 填充物質為3%OV-1時溫度為205℃；偵測器用火焰離子偵測器（FID） 。液相層析層析條件為'VERCOPAK' Inertsil ODS C18之層析管，偵測器為波長280nm之紫外光偵測器，流速：2 ml/min，移動相為MeOH：H20 =700:300 內含5.8g Sodium Dioctylsulfosuccinate 以磷酸調至pH3.2。本法適用於粉末、注射劑、錠劑、膠囊劑。

The methods of gas chromatography (GC) and high perfermance liquid chrommmatography (HPLC) were applied to separate and indentify heroin HCI, morphine HCI, pentazocine, cocaine HCI, caffeine, diphenhydramine HCI, prcacine HCI and chlorpheniramine maleate simultanously. lmpuities and inderference were excluded in these methods. The main purpose of this investigation was to developed a rapid, simple, relable and systemic methods to analysis there narcotic and its additives. The two GC and HPLC conditions are listed as follow: GC: condition 1. column: glass 6' x 1/4 x 2 mm (1)3% OV-1 on Chrom W 80-100 mesh (2)3% OV-17 on Chrom W80-100 mesh 2. detector: FID 3. temperature: (1)205℃ (2) program: 20 min intinial hold at 215'C then 15'C/min to 275'C HPLC: codition 1. column: 'VERCOP AK' Inertsil ODS C18 2. detector: UV 280 nm 3. flow rate: 2 ml/min 4. mbil phase: MeOH: H20; 700: 300 contains 5.8 g (0 .0013 M) sodiun dioctylsulfosuccinate adjust pH to 3.2 with H3 PO4.