台灣北部地區肉製品 Yersinia enterocolitica污染之調查

OCCURRENCE OF YERSINIA ENTEROCOLITICA IN PORK PRODUCTS FROM NORTHERN TAIWAN

自民國七十六年一月至四月，由臺灣北部地區抽購140件豬肉及其製品，進行 Yersinia enterocolitica 之檢驗，計有40件（28.6%）檢出Y. enterocolitica，包括豬肉檢出率為43.8% （28/64）；香腸為4.5% （2/44） ；水餃為50% （10/20）；火腿、肉醬、培根（燻肉）檢體則均未檢出，由此顯示在所有檢體中，以豬肉及水餃的污染最嚴重。根據變方分析顯示：Y. enterocolitica 檢出率受檢體種類及採樣時檢體保存狀態（冷藏、冷凍、非冷藏）影響。經血清學分析所分離的菌株，計17件檢體檢出其常感染血清型之 Y. enterocolitica，且以0:5血清型出現，頻率最高（10/17），0:8血清次之（6/17），0:3則僅檢出1件，0:5及0:8血清型之菌株，均分離自豬肉及冷凍水餃；0:3血清型之菌株則分離自冷凍豬肉且分離自17件檢體之菌株均擁有脂解酶，37℃ 自體凝集作用及37℃ 生長時需 Ca+2存在；其中分離自15件檢體之菌株屬 Nilehn's biotype 1及Wauter's biotype 2，另2件檢體之菌株屬 Nilehn's biotype 2及Wauter's biotype 2.。

One hundred and forty samples of retail pork products collected from Northern Taiwan during Jan. to Apr. 1987 were examined for the presence of Yersinia enterocolitica. The organism was recovered from 40 (28.6%) of these tested samples, including 28 (43.8%) from 64 raw pork samples, 2 (4.5%) from 44 sausage samples and 10 (50%) from 20 raviolis samples. The organism was not detected in hams, pates and bacons. With the analysis of variance, the detection rates were affected by sample items and storing conditions (frozen, refrigerated or non- refriger). Serotypable organisms were isolated from 17 samples, frequency rate for serotype 0：5, 0：8 and 0：3 were 10/ 17, 6/ 17 and 1/ 17 respectively. All 0：5, 0：8 strains were isolated from porks and raviolis while the 0：3 strain was from frozen pork. All of these serotypable Y. enterocolitica strains possessed lipase , and showed auto- agglutination and calcium -dependency when grew at 37℃ Furthermore, strains isolated from 15 samples were classified as Nilehn's biotype 1 and Wauter's biotype 2, the other were Nilehn's biotype 1 and W auter's biotype 2.