農產品中貝芬替殘留量標準檢驗方法之建立

ESTABLISHMENT OF STANDARD ANALYTICAL METHODS FOR DETERMINATION OF PESTICIDE RESIDUES IN CROPS—TESTS OF CARBENDAZIM

貝芬替（Carbendazim）屬氨基甲酸鹽系農藥，本研究發展出利用高效液相層析（high performance liquid chromatography, HPLC）檢驗作物中貝芬替殘留量之方法。貝芬替以丙酮自米、敏豆、木耳、小白菜、芒果、柑橘、楊桃中萃取出，經酸性氧化鋁（Acid aluminium cartridge）及碳十八（Cl8 Cartridge）層析管柱串聯淨化後以UV 280nm波長偵測之，結果添加0.25～3.0 ppm 檢體濃度之平均回收率為85.4～99.0%，最低檢出量在敏豆中為0.03 ppm。

Methods using high performance liquid chromatography ( HPLC ) to determine Carbendazim in crops have been developed. Carbendazim was extracted from crops, using acetone; it's analysis was determined by UV 280 nm after purification. Studies, performed at 0.25~3.0ppm fortification levels, showed recovery between 85.4~99.0%. The limit of detection of Carbendazim in kidney bean tested was 0.03ppm.