陳皮及其製劑之薄層層析鑑別

STUDIES ON THE THIN LAYER CHROMATOGRAPHIE IDENTIFICATION OF CITRI LEIOCARP AE EXOCARPINM AND THIS HERB IN SOME CHINESE HERBAL PREPARATIONS

陳皮（CITRI LEIOCARPAE EXOCARPIUM為陳久之橘皮亦稱陳橘皮，具健胃、整腸、止嘔、止吃逆、祛痰、止血及制菌等作用；被收載於中華民國中藥典範及日本藥局方；主要用途為成藥製劑之配方用藥，廣泛使用於芳香性健胃、消化藥及鎮咳去痰洩製劑。具有效成分為數種 flavonoids，特別是 hesperidin, narirutin, neohesperidir 及 nari ngin。以往鑑別陳皮藥材是以五官檢查，顯微鏡下觀察組切片及粉末，其後發展出化學呈色法及薄層層析法，但後者易受其他藥材共同存在之干擾，不適用於含陳皮之中藥製劑。故探求一簡易、方便和正確的含陳皮的中藥製劑之鑑別法，極為必要。本實驗先將市售陳皮藥材以薄層層析法分離出陳皮之特有成分及圖譜特徵，作為其共同性之探討。

In This experiment we developed three mobile-phase systems of T.L.C. for identifying citri leioearpae Exoearpium and this herb contained in chinese herbal preparations. The mobile-phase systems were tetrachlormethan-acetone (5 : 1), benzeneethylacetate (1 : 1) and chloroformmethanol-water (26 : 14 : 5 lower layer). with the mobile-phase systems we could easily separate Citri Leiocarpae Exocarpium in wu-ehi-san, Pii1.g-wei-san and Puchung-i-ehi-tang and clearly identify the spots corresponding to the constituents of Citri Leiocarpae Exocarpium with U.V. light ( 366nm ) or H2S04/ethanol (1 : 1) and ferric chloride T.S. spray reagents.