以逆被動乳膠凝集反應法鑑定能產對熱不穩定腸毒素之大腸桿菌

DETECTION OF HEAT-LABILE ENTEROTOXIN PRODUCED BY E. COLI WITH REVERSED PASSIVE LATEX AGGLUTINATION

自1987年6月至1988年3月間，由食品檢體中收受了103株大腸桿菌，以逆被動乳膠凝集反應（RPLA）測試，結果有84株為RPLA陽性反應19株為RPLA 陰性反應，此103菌株中有46株是來自食物中毒檢體的病原性大腸桿菌，其檢驗結果為34株RPLA呈陽性反應，12株為RPLA陰性反應。以美國FDA所贈4株產毒型大腸桿菌為對照組，測試結果為能產生對熱不穩定腸毒素之菌株，則RPLA為陽性反應。

From June 1987 to March 1988, 103 E. coli strains were collected from non-foodborne samples, fodborne samples, FDA (USA) etc. and analyzed for heat-labile E. coli enterotoxin (LT) by reversed passive latex agglutination (RPLA). The results showed that 84 strains were LT-positive, 19 strains were LT-negative. Forty six strains of enteropathogenic E. coli isolated from foodborne samples showed that 34 strains were LT- positive, 12 strains were negative. Four enterotoxigenic E. coli strains from FDA (USA) used as positive controls, can produce heat-labile E. coli enterotoxin and give positive results.