生鮮水產品中氣單胞菌之分離

Isolation of Aeromonas from Aquatic Foods in Taiwan

自民國82年8月至82年12月，分別在臺北地區之漁產批發中心、傳統市場、超級市場及其生鮮處理中心，共抽購285件市售生鮮水產品，分離氣單胞菌，結果檢出率為52%，其中以Aeromonas veroniilsobria DNA group 8、A. media-like DNA group 5A 及 A. hydrophila DNA group 1 佔最多，分別為25%、19%及13%。以95種生化試驗測定所有被分離出之298株氣單胞菌，其中11種試驗在各氣單胞菌間反應性差異很大，堪稱為關鍵性反應，可區分本研究分離出之8種氣單胞菌，即 glucose-1-phosphate、glucose-6-phosphate、bromosuccinic acid、D， L-lactic acid、r-aminobutyric acid、N-acetyl-D-galactosamine、propionic acid、cellobiose、α-hydroxybutyric acid、acetic acid 和β-hydroxybutyric acid。氣單胞菌之檢出率，在漁產批發中心之樣品中，養殖水產品顯然高於漁獲水產品（pO.05）。

From August to December 1993, 285 aquatic food samples were purchased from a wholesale center, traditional market, supermarket and fresh food processing center in the Taipei area, and were screened for Aeromonas, The total incidence was 52%. The incidence for A. veronii / sobria DNA group 8, A. media-like DNA group SA and A. hydrophila DNA group I, was 25%, 19%, and 13%, respectively, Ninty-five biochemical characteristics were determined for 298 Aeromonas isolates, and II tests exhibited major differences among the Aeromonas. These tests were assumed to differentiate 8 Aeromonas species, namely, glucose-1-phosphate, glucose- 6-phosphate, bromosuccinic acid, D, L-lactic acid, γ-aminobutyric acid, N-acetyl-Dgalactosamine, propionic acid, cellobiose, α-hydroxybutyric acid, acetic acid and β-hydroxybutyric acid. For samples collected from the wholesale center, theincidence of Aeromonas in the aqua-cultured samples was signiftcantly higher than that in the marine samples (p<0.05). For the marine samples, the incidence of Aeromonas was significantly higher for the samples from the supermarket or fresh food processing center compared to those from the wholesale center (p<0.05). For the aqua-cultured samples, no significant differences (p>0.05) were observed for samples collected from the different locations (wholesale center vs. traditional market; fresh food processing center vs. supermarket).