巿售梔子藥材中geniposide含量測定及其炮製之研究

QUANTITATIVE ANALYSIS OF GENIPOSIDE CONTENT IN GARDENIA FRUCTUS AND PROCESSING GARDENIA FRUCTUS CRUDE DRUG BY HPLC

利用高效液相層析法（HPLC），以去羫梔子苷（geniposide）為標準物質，於層析管：μ-Bondapak C18 （3.9mm I.D. x 30 cm），移動相：乙腈：水=15:85，檢測器 UV 238nm，0.1 AUFS，流速：0.6 ml/min 之條件下進行含量分析。結果市售30件梔子藥材之去羫梔子苷含量，山梔子：3.34～7.45%，水梔子：2.95～6.83%，且本實驗回收率均高達97.8%以上。同時，梔子藥材經炒黃、炒焦、炒皮、薑製及鹽製等方法炮製後，其去羫梔子苷之含量，除炒製者稍微增加而炭製者減少較明顯外，其餘均無顯著差別。

Determination of Geniposide content in Gardenia Fructus Crude drug by High performance liquid Chromatography [column: µ-Bondapak C18 (3.9 mm I.D. x30 em), Eluent:water:acetonitrile (85:15) , Detector: UV 238 nm, 0.1 AUFS, Flow rate: 0.6 ml/min]. The geniposide content in Gardenia Fructus crude drug are 2.95-7.45%. Gardenia Fructus processed by Carbonized, saute, salt-saute and zinger-saute, the geniposide content is no obvious difference between untreated and processed Gardenia Fructus except Carbonized.