飲料中古柯鹼檢驗方法之探討

GAS CHROMATOGRAPHIC METHOD FOR DETECTION COCAINE IN BEVERAGE

較常用於古柯鹼定量之方法，為高效液相層析法和氣相層析法兩種，比較此兩儀器對古柯鹼標準品之最低檢出量分別為100ng及4ng，故採用氣相層析儀分析之。又前處理時pH值及有機溶媒的選擇也影響著回收結果，添加標準品溶液於樣品中，使其最終濃度為0.2，0.5及1.0ppm，各濃度分別作三重覆，其平均回收率為82.8%，85.2%及88.1%，總平均回收率為85.4%，由結果顯示本檢驗方法可用於飲料中古柯鹼含量之檢驗，而其最低檢出量為0.05ppm。

A GC method was firstly compared with HPLC for cocaine content in a soft drink. The result indicated that the GC possessed higher sensitivity for cocaine hydrochloride. Its detecting limit was 4 ng compared to 100 ng by HPLC. Therefore the GC method was used in the further study. The cocaine solution were added to a soft drink at the final concentrations of 0.2, 0.5 and 1.0 ppm, the cocaine recoveries in the average of triplicate were 82.8, 85.2 and 88.1% respectively. The total average recovery was 85.0% the cocaine recovery was especially influenced by the conditions of pH and solvent during extration. The present GC method for cocaine showed a detection limit at 0.05 ppm.