HDPE塑膠袋水萃提升RAW 264.7 巨噬細胞之發炎反應

High Density Polyethylene (HDPE) Plastic Bags Water Extract Increase Inflammatory Response in RAW 264.7 Cells

塑膠袋是石化工業下重要的產物，已經成為日常生活不可或缺的必需品。在台灣為求食物攜帶方便，許多人會使用耐熱的HDPE塑膠袋盛裝熱食。但許多研究發現，塑膠袋加熱會釋出有毒物質，危害環境以及身體健康。我們的研究目的是希望探討耐熱的HDPE塑膠袋經過受熱後，是否會釋出引起巨噬細胞的發炎反應的物質。我們將塑膠袋水煮30分鐘，水萃取液配成DMEM培養基並且用來培養巨噬細胞。結果發現，加熱塑膠袋水溶液所製成的培養液，會刺激巨噬細胞分泌發炎相關細胞激素(IL-6與TNF-α)，以及釋出一氧化氮，並且表現一氧化氮合成酶以及COX-2，另外，也會誘導發炎訊息有關的NF-κB以及ERK1/2進行磷酸化。綜合以上的數據顯示，加熱後塑膠袋會釋出誘導巨噬細胞活化的物質，並透過NF-κB以及ERK1/2訊息表現，導致發炎的細胞激素與發炎媒介物釋放。

Plastic bag is an important product of the petrochemical industry, has become an indispensable daily necessities. In Taiwan, plastic bag is a very convenient way to carry hat food. However, heated plastic bags would release toxic substances for endangered the environment and the health of the people. This study aims to evaluate whether high density polyethylene (HDPE) plastic bags water extract could release inflammatory mediator in murine RAW264.7 macrophages. HDPE plastic bag boiled for 30 minutes, then DMEM cultured medium contained the extract water to culture macrophages. We found that the medium would induce proinflammatory cytokine productions (IL-6, TNF-α), and increased nitric oxide release in activated macrophages. We also found that the extracted medium increase iNOS and COX2 expression, and activated the phosphorylation of NF-κB and ERK1/2. These results suggest that water extract of HDPE plastic bag induce inflammatory effect by stimulated of NF-κB and ERK1/2 pathways that increased the levels of proinflammatory cytokines and mediators in RAW264.7 cells.