職業性錳暴露之生物偵測方法研究

Biological Monitoring of Occupational Manganese Exposure

錳是人體不可或缺的必要元素，但長期暴露在高濃度錳環境中，卻可能對健康造成不良影響。目前世界各國雖未將錳定為勞工健康檢查強制執行之項目，但因國內已有錳中毒的個案發生，因此為保障勞工健康，必須對錳做更進一步的研究。由於錳在生物體中的濃度一般均極低（為ng/g程度），因此須借助高靈敏的儀器技術才能有效地偵測。為符合勞工生物偵測所需之快速簡便的分析目標，本研究探求以石墨爐原子吸收光譜法（GFAAS）直接分析生物樣品中錳的可能性。初步的結果顯示，藉由基質修飾劑的添加及穩定化溫度平台爐（STPF）技術的運用，可有效地消除各種基質的干擾效應，達成快速準確的分析目標。本研究已建立一可適用於生物檢體中錳的參考分析方法，期能對錳之測量有所助益。

Manganese is widely distributed in nature and it is and essential trace element required for the maintenance of biological function in humans and other animals, but it is harmful to workers who are over exposed to Mn. Biological monitoring of Mn is not mandatory at present in the world, but the cases of chronic manganese intoxication in workers have been reported in Taiwan. Therefore further studies on the effects of Mn exposure on the workers' health is necessary. The concentration of Mn in biologic medium is very low (ng/g), so it can only be detected by instruments with high sensitivity. The purpose of the present study is therefore aiming at developing a simple, rapid and reliable methods to directly measure the concentration of Mn in biological samples. Our preliminary results showed that the matrix effect could be removed by STPF technique and matrix modifiers. The reference method for the biological monitoring of workers exposed to Mn was also proposed in this study.