蛹蟲草發酵液凍乾粉之28天重複劑量安全性分析

28-Day Subacute Oral Toxicity Assessment of Cordyceps Mmilitaris Mycelium in Rats

本研究針對蛹蟲草菌絲體發酵液粉(Cordceps militaris mycelium powder)進行28天亞急性餵食毒性評估。以口服途徑餵食SD大鼠，低、中及高三個劑量分別為1,000、2,000及3,000 mg/kg b.w.，另設空白對照組。每組各20隻，雌雄各半，大鼠經連續口服投予28天，每日進行臨床觀察，每週測量體重及攝食量。28天後進行臨床病理分析，解剖觀察有無明顯病變並進行組織病理檢測。結果顯示，SD大鼠經口餵食後，無明顯臨床症狀差異以及死亡現象發生，在試驗期間之體重變化及飼料攝取量，與對照組相較下均無明顯差異。各劑量組大鼠之尿液分析、血液學檢查、血清生化學檢查、臟器重量以及組織病理變化，與對照組亦皆無顯著性差異，因此，證明其無毒性顯示之劑量(no-observed-adverse-effect level, NOAEL)大於3,000 mg/kg b.w.。

The 28-day subacute oral toxicity of Cordyceps militaris mycelium powder produced by liquid fermentation technology was defined in this study. The SD rats were allocated to one of four groups, each consisting of ten males and ten females by treating different oral dosage from 0 (control), 1000, 2000 to 3,000 mg/kg (b.w.). Rats were orally administered for 28 days in a row. Daily observations such as obvious anatomical and pathological lesions and body weight measurement, and weekly food intake calculation were performed. All animals survived to the end of the study, and results showed no significant differences on clinical presentations including urinalysis, hematology, clinical chemistry, organ weight and histopathological changes. Therefore, no-observed--adverse-effect level (NOAEL) on C. militaris powder is considered to be at least greater than 3,000 mg/kg b.w..