勞工生物檢體汞物種偵測分析方法研究

Mercury Speciation in Urine and Blood of Workers

本研究採取暴露於汞的勞工之尿液和血液，利用自動流入分析系統配合冷蒸氣原子吸收光譜儀，發展出尿液和血液中汞物種分析的生物偵測方法，以探討勞工尿液和血液中無機汞及有機汞的暴露含量。在尿液樣品方面，以選擇性還原法偵測總汞量及無機汞量；在血液樣品方面，我們使用不同的消化方式，偵測總汞量及無機汞量。以上兩種的偵測方法有很好的精密度、回收率、偵測極限和準確率。結果顯示針頭、針筒和heparin，EDTA 抽血試管及未清洗的盛尿容器等的採樣容器，汞污染含量皆在0.06 μg/L 的偵測極限以下。對採樣時機而言，血液樣品在工作週期結束時採取；尿液樣品可隨機採樣。運送方面，只要以冰桶保持4℃運送檢體，則不致引起汞變化。尿液和血液直接儲存於-20℃的冰箱，並且盡可能於兩個㈪內完成分析。

A method of chemical speciation of mercury in urine and blood has been developed for biological monitoring of workers exposed to mercury. The automated flow injection analytical system coupled with cold vapor atomic absorption spectrophotometer (FIASCVAAS) was used to determine mercury vapor. In urine samples, the selective reduction was utilized to analyze total mercury and inorganic mercury. In whole blood samples, different digestion methods for the determination of total mercury and inorganic mercury were used. Both methods showed good precision, recovery, detection limit and accuracy. The concentration is below detection limit (0.06 μg/L) from needle contaminant, heparin and EDTA tubes and urine containers. The sampling time is suggested at the end of week for blood, and random for urine. On transferring samples, mercury concentration does not change in 4℃ ice chest. Urine and blood should be stored at -20℃ and analyzed within two months as soon as possible.