丁酮暴露生物偵測技術研究

Method Development for the Biological Monitoring of 2-Butanone Exposure

丁酮為國內工業界上常用之有機溶劑，而尿中丁酮濃度目前已被建議為勞工丁酮暴露之生物暴露指標。本研究中，將含有丁酮之尿液檢體以頂空技術（headspace technique）進行前處理，再以配備火燄游離偵測器之氣相層析儀進行定量分析。研究結果顯示最佳之前處理條件為加熱溫度80℃，加熱時間為60 分鐘，同時添加之碳酸鉀為2.50 克。六條同日與異日之檢量線相關係數均高於0.997，而各濃度之全變異係數（pooled coefficient of variantion）均小於3.50 %，檢量線濃度範圍為0.08 至51.20 μg/mL。當尿液樣品分別貯存於4 及-20℃時，其穩定性能維持一個月以上。本方法之偵測極限與可量化偵測極限分別為0.020 和0.065 μg/mL。添加回收率為97.49± 2.78 %。現場勞工真實尿液檢體測試結果亦顯示本分析方法確實能適用於工作場所勞工丁酮之暴露評估。因此本研究已成功的建立了一種能提供勞工丁酮暴露評估靈敏、簡單且可靠之生物偵測方法。

2-Butanone is a widely used organic solvent in industries. Urinary 2-butanone has been recommended as a good biomarker of exposure for 2-butanone. In this study, urine samples containing 2-butanone was treated by the head-space technique, then was analyzed by gas chromatography combined with a flame ionization detector. The optimal results were obtained when the heating temperature was 80℃, the heating time was 60 minutes, and when 2.5 gm of potassium carbonate was added in the head-space sample pretreatment procedure. The correlation coefficients of 6 inter-day and 6 intraday calibration curves of 2-butanone were greater than 0.997 and the pooled coefficients of variance were less than 3.5 % over the concentration range of 0.08 to 51.20 μg/mL. Urinary 2-butanone was stable for at least one months at 4 and -20℃. The limit of detection and the limit of quantitation were 0.020 and 0.065 μg/mL, respectively. The recovery of 2-butanone was 97.49± 2.78 %. Urine samples from exposed workers demonstrated that the proposed method is applicable to real samples in field study. It is therefore concluded that the proposed new method can provide a sensitive, simple, and reliable method for the biological monitoring of occupational exposure of 2-butanone.