螢光腐植酸與砷所引起動物組織的病理變化

Pathological Changes in Experimental Animals Induced by Fluorescence Humic Acid and Arsenics

以烏腳病地區井水粹取的螢光腐植物質注射實驗動物，實驗動物會產生類似烏腳病的病變，但以往學者認為砷才是造成烏腳病的原因，以砷做實驗，卻沒有動物成功產生症狀。此報告中，我們使用理化性質與烏腳病地區井水粹取物相似的購自Aldrich化學公司的螢光腐植酸，或螢光腐植酸加砷，用腹腔注射或口服，實驗動物在36-146天連續投藥後，成功產生類似鳥腳病病變。在注射螢光腐植酸加砷的一組，七隻小白鼠中有四隻產生症狀，在只注射螢光腐植酸的一組，七隻小白鼠中有兩隻產生症狀，但是雖未停止投藥，症狀卻在4-12天內完全消失，似乎小白鼠體內自有修復傷害的能力。病理切片研究也發現注射螢光腐植酸加砷的一組，組織傷害遠比只注射螢光腐植酸的一組嚴重，這也似乎表示螢光腐植酸的確有致病能力，而砷對螢光腐植酸的毒性則有加強作用。

Blackfoot disease can be reproduced in animal by injecting Fluorescence humic acid which was concentrated and purified from well water of blackfoot disease endemic area. Some other studies have also strongly suggested that the arsenic compounds in the well water was the cause of blackfoot disease, however, experimental studies with arsenic had not been reproducible.In this paper, we report that Aldrich Fluorescence humic acid with or without arsenic, gave experimental animals intraperitoneal injection or orally, in 36-146 days consecutively, can induce periphery vasculopathy mimic that of blackfoot disease. With Fluorescence humic acid mul arsenic injection, 4 out of 7 animals developed symptoms; with Fluorescence humic acid injection alone, 2 out of 7 animals developed symptoms, but all symptoms disappeared in 4-12 days even injections were continued. It seems, animals have the ability to recovery by themself. Pathological studies also showed more tissue damage in the group with Fluorescence humic acid and arsenic injection than the group with Fluorescence humic acid injection only. It means Fluorescence humic acid can induce tissue damage, and also, arsenic adds synergetic effcct on Fluorescence humic acid.