1,4-二氯苯的細胞毒性

Cytotoxicity of 1,4-Dichlorobenzene

及蛾類的燻煙劑，可能對生產或加工工廠操作員造成某些傷害。1,4-DCB 藉由受污染食物與飲水或皮膚接觸進入人體經代謝後，會產生的主要代謝物為2,5-二氯酚（2,5-Dichlorophenol, 2,5-DCP） 。與2,5-DCP比較後， 1,4-DCB 的毒性可反應在人類細胞株上，包含有人類肺癌 H1355 細胞株、人類肝癌 HepG2 細胞株、人類子宮頸癌 HeLa 細胞株與人類淋巴癌 Jurkat 細胞株，這些細胞株屬於 1,4-DCB 進入人體可能接觸的組織。 Trypan blue 染色法是常用於 30 分鐘毒性分析，而 caspase 3 / PI 偵測法則用於檢測細胞凋亡 (apoptosis) 與細胞壞死 (necrosis)。在人類肺癌 H1355 細胞株、人類肝癌 HepG2 細胞株、人類子宮頸癌 HeLa 細胞株與人類淋巴癌 Jurkat 細胞株毒性結果顯示，1,4-DCB 半致死率濃度分別為 1.02、1.02、1.42 與 1.18 mM。與 1,4-DCB 於人類淋巴癌 Jurkat 細胞株之毒性比較，顯示 2,5-DCP 之半致死率濃度高達 2.03 mM。進一步分析人類淋巴癌 Jurkat 細胞株經 1,4-DCB 處理 24 小時之細胞凋亡與細胞壞死結果，顯示 1,4-DCB 700 µM 有較高的細胞凋亡（大約 29%）。在 HeLa 粒線體膜電位偵測方面，500 µM 1,4-DCB 可造成 20% 細胞粒線體膜電位喪失。我們證實 1,4-DCB 與 2,5-DCP 均具細胞毒性 1,4-DCB 造成細胞死亡方面包含了細胞壞死與細胞凋亡。

1,4-dichlorobenzene (DCB), used as an ingredient in indoor deodorants, might be hazardous to workers in synthesizing or packaging facilities. 1, 4-DCB can be metabolized into 2,5-dichlorophenol (DCP) after entering the human body through the skin or orally via contaminated food and drink. The cytotoxicity of 1, 4-DCB, compared with 2, 5-DCP, was examined in human cell lines, including lung adenocaricinoma H1355 cells, hepatoma HepG2 cells, HeLa cells and leukemia Jurkat cells. Trypan blue staining assay was used to detect the cytotoxicity of 30-minute treatment, and caspase/PI detections was used to distinguish apoptosis and necrosis. The LD50S of 1, 4-DCB were 1.02, 1.02, 1.42 and 1.18 mM in lung adenocaricinoma H1355 cells, hepatoma HepG2 cells, leukemia Jurkat cells and HeLa cells. Compared with 1, 4-DCB, 2, 5-DCP had higher LD50 as 2.03 mM in leukemia Jurkat cells. The extent of apoptosis and necrosis in human leukemia Jurkat cells was analuzed after the cells had been treated with 1, 4-DCB for 24 hours. A very high percentage of apoptosis was gained (around 29%) in the presence of 1, 4-DCB above 700μM. When apoptosis was analyzed by a mitosensor to measure the mitochondria membrane potential in HeLa cells, 500μM 1, 4-DCB caused 20% of the cells to lose their mitochondria membrane potential.