| 中文摘要 |
本研究旨在發展以麩胺基硫（glutathione, GSH）為前還原劑配合高效能液相層析（high performanceliquid chromatography, HPLC）與流動注入氫化物產生原子吸收光譜法（flow injection-hydride generationatomic absorption spectrometry，FI-HGAAS）進行尿液中砷物種分析之方法。利用HPLC分離尿液中四個砷物種[三價砷（arsenite, As(III)）、五價砷（arsenate, As(V)）、單甲基砷（monomethylarsonic acid, MMAA）和雙甲基砷（dimethylarsinic acid, DMAA）]，以麩胺基硫（glutathione, GSH）為前還原劑將所有砷物種還原成As(III)，再以FI-HGAAS進行各個砷物種之定量分析。研究結果顯示，當溶解在0.01 mol/L HCl的10% GSH在40℃條件下與砷物種反應1小時，具有較其他前還原劑為佳的分析靈敏度，而且可在低酸和低硼氫化鈉（NaBH4）濃度下進行砷物種之分析。本研究所發展的方法之分析效能驗證結果如下：在2~10 μg/L範圍內，四個砷物種的檢量線都呈極佳的線性（r > 0.995）；理想的偵測極限，As(III)，As(V)，MMAA及DMAA分別為0.12 μg/L，0.15 μg/L，0.24 μg/L和0.12 μg/L；回收率（90 ~ 115%）、精密度（< 10%）和樣品穩定度（85 ~ 115%）皆在可接受範圍內。因此，本方法應具進行砷暴露勞工尿液中砷物種分析之可行性。 |
| 英文摘要 |
The aim of this study was to develop a method for urinary arsenic speciation using glutathione (GSH) as a pre-reductant by high performance liquid chromatography (HPLC) and flow injection-hydride generation atomic absorption spectrometry (FI-HGAAS). The HPLC was used to separate four urinary arsenic species: arsenite As(III), arsenate As(V), monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA). The GSH was employed as a pre-reductant before the detection of arsenic by FI-HGAAS. The results indicate that 10% of GSH dissolved in 0.01 mol/L HCl reacting with arsenic species at 40 °C for one hour was the optimal pre-reducing condition with better analytical sensitivity. In addition, the low concentrations of NaBH4 and HCl were available for the measurement of arsenic species by the proposed method. A consistent linearity (r > 0.995) was observed for each of the four arsenic species within the linear range of 2 to 10 μ g/L. The detection limits of 0.12, 0.15, 0.24 and 0.12 μ g/L were obtained for As(III), As(V), MMAA and DMAA, respectively. The recovery (90 ~ 115%) and precision (< 10%) were in the acceptable range. It is, therefore, concluded that the developed method is feasible for urinary arsenic speciation in arsenic-exposed workers. |
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