探討碳酸氫鈉溫泉清除氫氧自由基的作用

Hydrogen Carbonate Hot Spring Water Plays the Role of Hydroxyl Radicals Scavenger

溫泉具有肌膚美容和保健的功效，但詳細的作用機制仍有待更多的科學研究證據來闡釋。本研究以位於台灣中央山脈衍生的碳酸氫鈉溫泉為研究素材，溫泉取樣處為高雄縣境內的七坑溫泉，研究採Fenton反應系統來衍生氫氧自由基（OH），及配合氫氧自由基與水楊酸反應的生成產物2,3-DHBA和2,5-DHBA的濃度，來探討碳酸氫鈉溫泉吸收氫氧自由基的能力。結果顯示，溫泉原液相較Milli-Q水能有效抑制2,3-DHBA和2,5-DHBA生成，平均值分別為91.3±0.59%及95.1±0.48%；1倍稀釋溫泉抑制效果，平均值分別為89.2±1.35%及94.4±0.96%；10倍稀釋溫泉抑制效果，平均值分別為83.6±1.24%及90.4±1.33%；100倍稀釋溫泉抑制效果，平均值分別為68.3±2.17%及70.1±3.28%；1,000倍稀釋溫泉抑制效果，平均值分別為29.8±3.23%及32.1±4.37%。以10倍稀釋溫泉水調製成10 mM的褪黑激素溶液，原10倍稀釋溫泉對2,3-DHBA和2,5-DHBA生成的抑制效果，平均值分別為83.6±1.24%及90.4±1.33%，混合液對2,3-DHBA和2,5-DHBA生成的抑制效果，平均值分別為92.7±2.89%及91.8±1.54%，可見褪黑激素與溫泉混合後，褪黑激素吸收氫氧自由基的能力有明顯被增強的作用。總結本研究結果顯示，七坑碳酸氫鈉溫泉明顯具有清除氫氧自由基的作用，原液效果優於100 mM維生素C和100 mM褪黑激素對氫氧自由基的清除作用，但其作用受維生素C所干擾而下降，與褪黑激素合用則有增強褪黑激素清除氫氧自由基的作用，結果可提供作為解釋碳酸氫鈉溫泉浸泡的人體保健功效。

The unpollution of hydrogen carbonate spring waters （HCSW） taken from Chi-Keng （Kaohsiung, Taiwan） were analyzed for the scavenging effect of hydroxyl radical. These conditions are favorable for the generation via Fenton chemistry of the hydroxyl radical that was measured by HPLC using salicylic acid as a probe. The results indicate that the HCSW may play the role of hydroxyl radicals scavenger in different concentrations （0, 1, 10, 100, 1000 diluted spring water）. Compared with milli-Q water groups, the different diluted spring water groups decreased 2,3-DHBA and 2,5-DHBA levels by 91.3±0.59% and 95.1±0.48% in the original spring water group, by 89.2±1.35% and 94.4±096% in 1:1 diluted spring water group, by 83.6±1.24% and 90.4±1.33% in 1:10 diluted spring water group, by 68.3±2.17% and 70.1±3.28% in 1:100 diluted spring water group, by 29.8±3.23% and 32.1±4.37% in 1:1000 diluted spring water group, respectively. Two antioxidants, vitamin C and melatonin were played a potent role of hydroxyl radical scavenger. The melatonin scavenging effect was increased by the HCSW in melatonin-spring water mixed solution. However, the low concentration of vitamin C （≤10 mM） in vitamin C-spring water mixed solution was played a pro-oxidant effect that was against the effect of scavenging hydroxyl radicals produced by carbonated spring water. The results suggested that hydrogen carbonate hot spring water plays the role of hydroxyl radical scavenger.