高效液相層析法對小青龍湯中七種成分同時分析之研究

Simultaneous Analysis of Seven Components in Hsiao-Ching-Lung-Tang Preparation by High Performance Liquid Chromatography

使用HPLC進行小青龍湯之成分分析，開發出包含白芍中paeoniflorin，paeonol、桂枝中cinnamic acid，cinnamaldehyde、甘草中glycyrrhizin 及五味子中gomisin A，schizandrin 等七種指標成分同時定量分析方法。小青龍湯之檢品溶液通過保持在30°C恒溫之HPLC層析管（Inertsil ODS-2, 4.6 mm I.D.°—250 mm），移動相採用5%及60% acetonitrile之混和溶液，並用磷酸調整pH值為2.8，進行梯度沖提法，以1.0 mL ／分之流速沖提。七種指標成分之檢測使用UV偵測器，偵測波長設定245 nm。本法之回收率，同日間及異日間的變異係數均在5%以下，相對誤差各低於4.2%及4.1%，回收率在94.7~122.3%之間。這個分析法對於小青龍湯製劑中七種指標成分是安定且值得信賴之定量法。To enhance quality control in “Hsiao-Ching-Lung-Tang”, a simultaneous determination method using High Pressure Liquid Chromatography (HPLC) on seven marker substances was developed. The marker substances were paeoniflorin and paeonol from Paeoniae Radix; cinnamic acid and cinnamaldehyde from Cinnamomi Ramulus; glycyrrhizin from Glycyrrhizae Radix; and gomisin A and schizandrin from Schizandrae Fructus. Sample extracts were analyzed using a reverse-phase column (Inertsil 5 ODS-2, 4.6 I.D. °— 250 mm.) at 30°C and then eluted with a solvent of mixture of 5% and 60% acetonitrile aqueous solution (adjusted to pH 2.8 with phosphoric acid) in gradients at a flow-rate of 1.0 mL/min, and were detected at UV245 nm. Relative coefficients of variations of intra- and inter-day analysis were less than 5% and the relative errors were below 4.2% and 4.1%, respectively. All the recoveries were between 94.7 and 122.3%. This method could be applied for the simultaneous determination of seven marker substances in “Hsiao-Ching-Lung-Tang” and as a comparison to commercial preparations.