人參皂苷Rd及人參萃取物對C57BL/6JNarl鼷鼠Cyp1a, Cyp2b, Cyp2c, Cyp2e1與Cyp3a 活性之影響

Effects of Ginsenoside Rd and Panax Ginseng Extract on Cyp1a, Cyp2b, Cyp2c, Cyp2e1 and Cyp3a Activities in C57BL/6JNarl Mice

"人參（根）被廣泛地使用於食物及傳統中藥治療，尤其是在亞洲。改變肝微粒體細胞色素P450 活性是造成草藥- 藥物交互作用之主要原因之一。然而，對雄性C57BL/6JNarl鼷鼠，每天口服投與劑量高至50 毫克/公斤的人參皂苷Rd，連續處理7 天，不影響鼠肝微粒體7-ethoxyresorufinO- 去乙基、pentoxyresorufin O- 去烷基、tolbutamide 氫氧化、nitrosodimethylamineN- 去甲基、nifedipine氧化活性，這些活性分別為Cyp1a2、Cyp2b、Cyp2c、Cyp2e1、Cyp3a的指標活性。口服投與50 毫克/公斤的人參乙醇萃取物，連續處理7 天對鼠肝微粒體的這些活性亦無影響。這些結果缺乏證據顯示在所測定的條件下，人參皂苷Rd 或人參萃取物可以影響細胞色素P450 活性。The roots of Panax ginseng has been widely used in foods and the prescriptions of traditional Chinese medical care, especially in Asia. Alteration of liver microsomal cytochrome P450 activity is one of the primary factors causing herb-drug interaction. However, administration of a daily dose up to 50 mg ginsenoside Rd/kg to male C57BL/6JNarl mice for 7 consecutive days did not affect liver microsomal 7-ethoxyresorufin O-deethylation, pentoxyresorufin O-dealkylation, tolbutamide hydroxylation, nitrosodimethylamine N-demethylation, and nifedipine oxidation activities, which were marker activities of Cyp1a, Cyp2b, Cyp2c, Cyp2e1, and Cyp3a, respectively. Treatment with 50 mg/kg of ethanol extract of P. ginseng did not affect these activities either. These results indicate no evidence for the alteration of P450 activities by either ginsenoside Rd or P. ginseng extract under the treatment regimen in this study. "