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篇名
血紅密孔菌菌絲體之抗發炎活性成分研究
並列篇名
Anti-Inflammatory Principles of Cultivated Pycnoporus Sanguineus
作者 盧美光林雪娥徐鳳麟林雲蓮
中文摘要
血紅密孔菌 (Pycnoporus sanguineus (Fr.) Murr) 為一種木材腐朽菌,被用於保健及機能性食品。本研究以脂多醣 (lipopolysaccharide, LPS) 誘導小鼠微膠小細胞株, BV-2細胞產生一氧化氮(nitric oxide, NO) 的抗發炎模式,篩選人工培養之血紅密孔菌之菌絲體的活性成分。依此生物活性導向,進行成分分離純化,共得到四個麥角甾醇衍生物,分別為:ergosta-7,22-dien-3β-ol(1), 3β,5α-dihydroxyergosta-7,22-dien-6-one (2), 3β,5α,9α-trihydroxyergosta-7,22-dien-6-one (3) 及3β,5α,6α-trihydroxyergosta-7,22-diene (4)。其中化合物2,3和4三個化合物是首次自血紅密孔菌中分離得到之已知化合物。化合物1對LPS誘導之微神經膠細胞株有顯著的抑制,在15 μM濃度有43.5 % 的抑制率。Pycnoporus sanguineus is a parasitic fungus found on dead broadleaf trees and used as a nutraceuticals and functional food. LPS-induced NO production in a mouse microglial cell line, BV-2 cells was used as inflammatory model to screen neuro-protective compounds from the cultivated mycelia of P. sanguineus. Bioassay-guided fractionation was led to the isolation of four ergostane skeleton type steroids, including ergosta-7,22-dien-3β-ol (1), 3β,5α-dihydroxyergosta-7,22-dien-6-one (2), 3β,5α,9α-trihydroxy-ergosta-7,22-dien-6-one (3), and 3β,5α,6α-trihydroxy-ergosta-7,22-diene (4) from mycelia of cultivated P. sanguineus. Among that, compounds 2, 3, and 4 were newly isolated in this species. Compound 1 showed significantly inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV-2 cells, at a concentration of 15 μM in the value of 43.5%.
起訖頁 75-83
關鍵詞 過敏性鼻炎中醫辨證分型腎氣虛年齡Allergic rhinitisTraditional Chinese medicine patternsTCM patternsKidney qi deficiencyAge
刊名 中醫藥雜誌  
期數 201012 (21:3-4期)
出版單位 衛生福利部國家中醫藥研究所(原:國立中國醫藥研究所)
該期刊-下一篇 穿心蓮乙素 (andrographolide) 經由抑制iNOS和gp91phox/NADPH oxidase 2 (NOX2) 的表現保護缺血型中風大鼠腦組織
 

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