| 中文摘要 |
血紅密孔菌 （Pycnoporus sanguineus （Fr.） Murr） 為一種木材腐朽菌，被用於保健及機能性食品。本研究以脂多醣 （lipopolysaccharide, LPS） 誘導小鼠微膠小細胞株, BV-2細胞產生一氧化氮（nitric oxide, NO） 的抗發炎模式，篩選人工培養之血紅密孔菌之菌絲體的活性成分。依此生物活性導向，進行成分分離純化，共得到四個麥角甾醇衍生物，分別為：ergosta-7,22-dien-3β-ol（1）, 3β,5α-dihydroxyergosta-7,22-dien-6-one （2）, 3β,5α,9α-trihydroxyergosta-7,22-dien-6-one （3） 及3β,5α,6α-trihydroxyergosta-7,22-diene （4）。其中化合物2，3和4三個化合物是首次自血紅密孔菌中分離得到之已知化合物。化合物1對LPS誘導之微神經膠細胞株有顯著的抑制，在15 μM濃度有43.5 % 的抑制率。Pycnoporus sanguineus is a parasitic fungus found on dead broadleaf trees and used as a nutraceuticals and functional food. LPS-induced NO production in a mouse microglial cell line, BV-2 cells was used as inflammatory model to screen neuro-protective compounds from the cultivated mycelia of P. sanguineus. Bioassay-guided fractionation was led to the isolation of four ergostane skeleton type steroids, including ergosta-7,22-dien-3β-ol (1), 3β,5α-dihydroxyergosta-7,22-dien-6-one (2), 3β,5α,9α-trihydroxy-ergosta-7,22-dien-6-one (3), and 3β,5α,6α-trihydroxy-ergosta-7,22-diene (4) from mycelia of cultivated P. sanguineus. Among that, compounds 2, 3, and 4 were newly isolated in this species. Compound 1 showed significantly inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV-2 cells, at a concentration of 15 μM in the value of 43.5%. |
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