十全大補藥酒成分分析之研究

Studies on the Component Analysis in Tonic Wine Preparation of Shyr-Chyuan-Dah-Buu-Yaw-Jyo

使用HPLC 進行十全大補藥酒之成分分析，開發出包含當歸、川芎中ferulic acid 、白芍中paeoniflorin 、甘草中glycyrrhizin 、肉桂中cinnamic acid ，cinnamaldehyde 及黃耆中calycosin 等六種指標成分之多成分同時定量分析方法。同時以不同製程條件探討製程對十全大補藥酒品質之影響。十全大補藥酒之試料通過保持在30 °C 恒溫之HPLC 層析管（Inertsil ODS-2, 4.6 mm i.d. ×250 mm ），移動相採用10% 及60% acetonitrile （各用磷酸調整pH 值為2.8 ）之混合溶液，進行梯度沖提法，以1.0 mL/ 分之流速沖提。六種指標成分之檢測使用UV 偵測器，偵測波長設定240 nm 。本法之回收率，同日間及異日間的變異係數均在5% 以下，回收率在92.30~115.00% 之間。這個分析法對於十全大補藥酒製劑中六種指標成分是安定且值得信賴之定量法。A facile HPLC method for the resolution and quantitative measurement of six marker substances, the active ingredients in tonic wine preparation of Shyr-Chyuan-Dah-Buu-Yaw-Jyo, was established under the gradient elution in the reversed-phase mode. These marker substances included ferulic acid (Angelicae Sinensis Radix and Cnidii Rhizoma), paeoniflorin (Paeoniae Radix), glycyrrhizin (Glycyrrhizae Radix), cinnamic acid, cinnamaldehyde (Cinnamomi Cortex), and calycosin (Astragali Radix). Different manufacture conditions were performed to evaluate quality of Shyr-Chyuan-Dah-Buu-Yaw-Jyo. Extracted samples were analyzed with reversed-phase column (Inertsil 5 ODS-2, 4.6 i.d. × 250 mm) at 30 °C and eluted with a mixture of 10% acetonitrile and 60% acetonitrile (each adjusted to pH 2.8 with phosphoric acid) aqueous solution in gradient manner at a flow-rate of 1.0 mL/min, and detected at 240 nm. Relative coefficients of variations of intra- and inter-day analysis were less than 5%. All the recoveries were 92.30~115.00%. This method could be applied for the simultaneous determination of six marker substances in “Shyr-Chyuan-Dah-Buu- Yaw-Jyo”.