| 中文摘要 |
冬蟲夏草（Cordyceps sinensis, C. sinensis） 為大家熟知可以調節人體免疫系統的中草藥。樹突狀細胞（dendritic cells ，DC ）是很重要的抗原呈現細胞（antigen presenting cell ），其成熟所引發T 細胞的反應，在癌症的免疫治療上扮演了很重要的角色。我們研究冬蟲夏草提取液是否影響取自人類週邊血液單核球（peripheral blood monocyte ，PBMC ）所培養之樹突狀細胞的成熟，並且測量細胞激素的分泌。來自健康捐贈者血液的單核球與顆粒球– 巨噬細胞群落刺激因子（GM-CSF ）和白細胞介素4 （IL-4 ）共同培養6 天，然後加入熱水提取的冬蟲夏草培養2 天。經冬蟲夏草提取物的刺激，細胞表面分子CD80 、CD83 、CD86 和HLA-DR 相較於控制組皆有明顯的增加，其RMFI （ratio of mean fluorescence intensity ）分別為1.70 ± 0.32 ，1.20 ± 0.01 ，2.29 ± 0.29 和1.44 ± 0.23 （n = 5, p < 0.05 ）。此外，與異體T 細胞共同培養，冬蟲夏草提取物亦顯著地增加IL-12 p40 （256.8 ± 185.4 pg/mL ，控制組為98.2 ± 46.73 pg/mL ）和IFN-γ （311.1 ±156.4 pg/mL ，控制組為22.5 ± 35.1 pg/mL ）的製造。這些發現顯示，冬蟲夏草提取物對於人類樹突狀細胞來說是很有潛力的刺激物，且其作用可能與調節Th1 細胞的功能有關。Cordyceps sinensis (C. sinensis) is well known for its modulating effect on host immune system. As dendritic cells (DC) are professional antigen-presenting cells (APC) that play a key role in cancer immunotherapy, the maturation of DC is critical to the induction of T-cell responses. We investigated the effect of C. sinensis extract on the maturation of human monocyte-derived DC and the secretion of cytokines. Peripheral blood mononuclear cells (PBMC) from healthy donors were cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 6 days, then stimulated by hot-water extract of C. sinensis for 2 days. Stimulation with C. sinensis extract increased cell surface expression, and the RMFI (ratio of mean fluorescence intensity) of CD80, CD83, CD86 and HLA-DR are 1.70 ± 0.32, 1.20 ± 0.01, 2.29 ± 0.29 and 1.44 ± 0.23 (n = 5, p < 0.05), respectively. Moreover, when co-cultured with allogeneic T cells, C. sinensis extract caused the significant production of IL-12 p40 (256.8 ± 185.4 pg/mL, control group is 98.2 ± 46.73 pg/mL) and IFN-γ (311.1 ± 156.4 pg/mL, control group is 22.5 ± 35.1 pg/mL). In conclusion, these findings suggest that C. sinensis extract is a potent stimulator of DC and may be related to modulation of Th1 cells functions. |
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