月旦知識庫
  1. 熱門:
 
首頁 臺灣期刊   法律   公行政治   醫學   財經   社會學   教育   其他 大陸期刊   核心   非核心 DOI文章
查看詳細全文
篇名
冬蟲夏草提取液促進人類單核球轉化之樹突狀細胞的成熟與功能
並列篇名
Cordyceps Sinensis Extract Promotes Phenotypic and Functional Maturation of Human Monocyte-Derived Dendritic Cells
作者 魏麒容郭敏玲楊榮季張雪玲謝佳蓉
中文摘要
冬蟲夏草(Cordyceps sinensis, C. sinensis) 為大家熟知可以調節人體免疫系統的中草藥。樹突狀細胞(dendritic cells ,DC )是很重要的抗原呈現細胞(antigen presenting cell ),其成熟所引發T 細胞的反應,在癌症的免疫治療上扮演了很重要的角色。我們研究冬蟲夏草提取液是否影響取自人類週邊血液單核球(peripheral blood monocyte ,PBMC )所培養之樹突狀細胞的成熟,並且測量細胞激素的分泌。來自健康捐贈者血液的單核球與顆粒球– 巨噬細胞群落刺激因子(GM-CSF )和白細胞介素4 (IL-4 )共同培養6 天,然後加入熱水提取的冬蟲夏草培養2 天。經冬蟲夏草提取物的刺激,細胞表面分子CD80 、CD83 、CD86 和HLA-DR 相較於控制組皆有明顯的增加,其RMFI (ratio of mean fluorescence intensity )分別為1.70 ± 0.32 ,1.20 ± 0.01 ,2.29 ± 0.29 和1.44 ± 0.23 (n = 5, p < 0.05 )。此外,與異體T 細胞共同培養,冬蟲夏草提取物亦顯著地增加IL-12 p40 (256.8 ± 185.4 pg/mL ,控制組為98.2 ± 46.73 pg/mL )和IFN-γ (311.1 ±156.4 pg/mL ,控制組為22.5 ± 35.1 pg/mL )的製造。這些發現顯示,冬蟲夏草提取物對於人類樹突狀細胞來說是很有潛力的刺激物,且其作用可能與調節Th1 細胞的功能有關。Cordyceps sinensis (C. sinensis) is well known for its modulating effect on host immune system. As dendritic cells (DC) are professional antigen-presenting cells (APC) that play a key role in cancer immunotherapy, the maturation of DC is critical to the induction of T-cell responses. We investigated the effect of C. sinensis extract on the maturation of human monocyte-derived DC and the secretion of cytokines. Peripheral blood mononuclear cells (PBMC) from healthy donors were cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 6 days, then stimulated by hot-water extract of C. sinensis for 2 days. Stimulation with C. sinensis extract increased cell surface expression, and the RMFI (ratio of mean fluorescence intensity) of CD80, CD83, CD86 and HLA-DR are 1.70 ± 0.32, 1.20 ± 0.01, 2.29 ± 0.29 and 1.44 ± 0.23 (n = 5, p < 0.05), respectively. Moreover, when co-cultured with allogeneic T cells, C. sinensis extract caused the significant production of IL-12 p40 (256.8 ± 185.4 pg/mL, control group is 98.2 ± 46.73 pg/mL) and IFN-γ (311.1 ± 156.4 pg/mL, control group is 22.5 ± 35.1 pg/mL). In conclusion, these findings suggest that C. sinensis extract is a potent stimulator of DC and may be related to modulation of Th1 cells functions.
起訖頁 47-55
關鍵詞 李東垣蘭室秘藏學術思想脾胃元氣陰火學說LiDong-yuanLan-Shi-Mi-CangThoughtYuan-Qi-Yin-Fire hypothesis
刊名 中醫藥雜誌  
期數 200503 (16:1期)
出版單位 衛生福利部國家中醫藥研究所(原:國立中國醫藥研究所)
該期刊-上一篇 還少丹於減緩老化之應用研究
該期刊-下一篇 樟芝(Antrodia camphorota, strain CCRC-35396)培養液中具抗B 型肝炎病毒活性成分
 

新書閱讀



最新講座


優惠活動




讀者服務專線:+886-2-23756688 傳真:+886-2-23318496
地址:臺北市館前路28 號 7 樓 客服信箱
Copyright © 元照出版 All rights reserved. 版權所有,禁止轉貼節錄