闊葉大豆（金門產一條根）水粗萃取物對大鼠28天餵食毒性作用

EFFECTS OF 28-DAY’S REPEATED ORAL ADMINISTRATION OF CRUDE AQUEOUS EXTRACTS OF GLYCINE TOMENTELLA HAYATA ON RATS

本實驗主要探討闊葉大豆（金門產一條根）水粗萃取物連續經口投予28 天對雌、雄大鼠的毒性。闊葉大豆水粗萃取物的劑量為每天投予1 、2 或3 g/kg 。血液學檢查：雌鼠3 g/kg 組出現平均紅血球容積增加的情形。血清生化學檢查：雌鼠3 g/kg 組血中鈉、鉀離子濃度及麩氨酸草乙酸轉氨　活性增加，但總膽固醇、總蛋白，白蛋白、球蛋白、總膽紅素、肌酸酣及鎂離子濃度降低。雌鼠2 g/kg 組也出現總膽紅素及鎂離子濃度下降的情形。尿液分析：雄鼠與雌鼠3 g/kg 組尿液鉀璃子量增加，雌鼠3 g/kg 組蛋白尿增加。雄鼠3 g/kg 組精囊重量萎縮，但並無實質上的病理組織變化。大鼠連續投予討闊葉大豆水粗萃取，安全劑量在2 g/kg 以下。Toxicity study was carried out by administration orally daily doses of 0, 1, 2 and 3 g/kg of crude aqueous extracts of Glycine tomentella Hayata (GTE) to groups of male and female rats for 28 days. In hematology, female rats given 3 g/kg showed an increase in mean corpuscular volume (MCV). In blood chemistry, an increased levels of sodium, potassium and activities of glutamic oxalacetic transaminase (GOT) were observed in female rats of 3 g/kg dose group. Female rats given 3 g/kg showed a decrease in concentration of total cholesterol, total protein, albumin, gloublin, total bilirubin and magnesium. Female rats given 2 g/kg also showed a decrease in concentration of total bilirubin and magnesium. Urinalysis showed an increase in Na+ level in both male and female rats of 3 g/kg dose group. Increased urinary protein was observed in female rats of the 3 g/kg dose group. Male rats given 3 g/kg showed an increase seminal vesicle weight. However, no accompanying histopathological changes were observed. In conclusion, the toxicological noobserved levels in rats were thought to be 2 g/kg or below.