臺灣產崗脂麻（Helicteres angustifolia L.）之藥理活性評估

EVALUATION OF THE PHARMACOLOGICAL EFFECTS ON HELICTERES ANGUSTIFOLIA L. IN TAIWAN

崗脂麻（Helicteres angustifolia L.）為梧桐科（Sterculiaceae）植物，其根在臺灣市場品中以其消腫解毒之功，應用於喉痛、扁桃腺炎而常被當成山豆根來使用。實驗結果顯示其甲醇粗抽物對小鼠口服給藥之致死量（LD50）為2.54 g/kg。並發現其甲醇粗抽物及其不同有機溶媒萃取層對A-549、HT-29、KB-16 及P-388 等四種癌細胞均有明顯之抑制作用，以乙酸乙酯萃取層（Ha-E）對HT-29之作用最強。對發炎細胞之作用，甲醇粗抽物及各萃取層對fMLP 所誘發嗜中性白血球的去顆粒作用中，對β-glucuronidase 釋出具顯著之抑制作用（P < 0.01）；乙酸乙酯及正丁醇萃取層（Ha-B）之IC50分別為16.7 及56.3 μg/ml。此二層萃取物對lysozyme 之釋出亦具抑制作用。以compound 48/80誘發肥滿細胞去顆粒作用中，二氯甲烷萃取層（Ha-C）在10 μg/ml 之濃度，正丁醇萃取層（Ha-B）及水層（Ha-W）在30 μg/ml 之濃度時，對histamine 釋出有抑制作用，但對β-glucuronidase 之釋出則無抑制作用。Helicteres angustifolia L., family of Sterculiaceae, is a folk medicine commonly adulterated or substituted as Shan-Don-Gen (Sophora Subprosprata Radix) in Taiwan market. Due to analgesic, antipyretic, cytotoxic and anti-inflammatory effects, it has been used to treat swelling, sorethroat and acute pharyngitis. The present study showed that the LD50 of the methanol extract of the root of this plant on mice was 2.54 g/kg (p.o.). The crude methanol extract and its fractions partitioned by nhexane, chloroform, ethyl acetate, n-butanol and water exhibited marked cytotoxic effect against A- 549, HT-29, KB-16, and P-388 cell lines, respectively. Among them, the ED50 of the EtOAc layer was 0.581 μg/ml against HT-29 cell line. The crude methanol extract and fractions also showed significant inhibitory effects against β-glucuronidase release on neutrophil degranulation induced by fMLP. Fractions of ethyl acetate and n-butanol layer showed the best anti-inflammatory effect with IC50 of 16.7 and 56.3 μg/ml, respectively. They also showed similar inhibitory effects against lysozyme release in this experiment. In the mast cell degranulation induced by compound 48/80 experiments, chloroform layer at 10 μg/ml, EtOAc and water layer at 30 μg/ml, exhibited remarkable antiallergic effect against histamine release, but they did not show the effects on the release of β-glucuronidase.