The purpose of study was to investigate the therapeutic effects of Gypenoside Formulas (GFs) on acute hepatotoxicityh induced by CCL?? and D-galactosamine (D-GalN) in rats. The experimental animals were divided into 6 gourps of each 8 rats. A group was a normal control and B group was CCL induced hepatotoxicity. Group C, D and E were treated with GF-1 (Gypenoside), GF-2 (Gypenoside + bifenbate), and GF-3 (Gypenoside + bifenbate + dan-shen) respectively. Group F was treated with reference drug (silymarin).
The results demonstrated that the GFs and the reference drug were markedly decreased the biochemical elevated serum levels of SGOT and SGPT in the acute CCl?? and D-GalN induced hepatotoxicity. Further analysis of the drug-treated groups indicated that the effects of GF-2 and GF-3 were the most potent with an obvious hepatoprotection. However, it was not statistical significance among the drug treated groups for D-GalN induced hepatotoxicity. The GFs and silymarin treated the CCL?? hepatotoxicity, the pathological lesion showed a various improvement by Jonker's semiquantitative analysis. The GF-1 decreased liver cell inflammation and mitosis, the GF-2 improved the hepatocytes inflammationj, mitosis, fatty change and decreased the bile duct proliferation, the GF-3 improved inflammatory cells infiltration, cellular necrosis and fatty change had a statistically significant effects. The drug groups treated the D-GalN hepatotoxicity according to the Jonker's pathological semiquantitative analysis, the results revealed that the GFs had an obviously various effectiveness. The GF-1 was significantly hyaline degeneration, fatty change, cell necrosis and bile ducts proliferatin. And the GF-3 improved the hyaline degeneration, fatty change and bile ducts proliferation with statistical significance. In this study the GFs and silymarin were not statistical significance on anti-hepatocyte inflammation induced hy D-GalN hepatotoxicity.