川芎嗪於大白鼠腦部各區之濃度分佈研究

Regional Brain Distribution of Tetramethylpyrazine in Rat

本研究應用一套靈敏、可靠的高效液相層析法，來定量川芎嗪於大白鼠腦組織各區濃度。以甲醇與水（50:50，v/v，pH3.0以正磷酸調整）當作移動相，使用碳18的道相分離管柱（4.6×250mm，粒經5μm)，流速為每分鐘1ml進行待測物分離，並以紫外光檢測器280nm為偵測波長。腦組織最低可定量偵測極限為0.1μg/g，每次取100μg來作分析。實驗以10mg/kg川芎嗪的劑量靜脈注射15分鐘後，迅速將大白鼠斷頭犧牲，並將其腦組織分成六個部分（划崗皮質、腦榦、紋狀體、海馬迴、，小腦及中腦），分析川芎嗪在各區之濃度，結果顯示，投藥御街組織六個部分的川芎嗪的濃度呈現均勻的分佈，且周邊血漿川芎嗪的濃度約高於腦組織五倍，靜脈注射後約17%的川芎嗪可以通過血腦障壁。

We used a rapid and reliable high-performance liquid chromatographic method for determination of tetramethylpyrazine in rat brain. The separation was achieved on a Cosmosil 5C18 column (4.6 x 250 mm, particle size 5 um). The mobile phase consisted of methanoi-water (50:50, v/v, pH 3.0 adjusted by orthophosphoric acid) at a flow rate of 1.0 mL/min. Tetramethylpyrazine was monitored at a wave- length of 280 nm. The lower limit of quantification in brain tissue was O.1 ug/g, and only a small amount of brain tissue (100 ug) was required for analysis. Concentrations of tetramethylpyrazine in various regions of the brain (cerebral cortex, brainstem, striatum, hippocampus, cerebellum and mid- brain) were not significantly different at 15 min afteradministration (10 mg/kg, i.v.). Mean concentra- tion of the tetramethylpyrazine in plasma was approximately five times greater than in brain tissue. In additional analysis, about 17% of tetramethylpyrazine may penetrate blood-brain-barrier.