九節木之活血化瘀活性分析

Analysis on the Vasoactive-Antithrombotic Effect of Psychotria Rubra

張仲景於金匱要略中首先提出「瘀血」病名，許多研究指出，現在醫學的心血管、婦科、免疫等系統之疾病皆可用「活血化瘀」方藥治療，而血小板凝集實驗是證明藥物是否具有活血化瘀研究最重要的方法。因此，本研究以九節木 [ Psychotria rubra (Lour.)Poir. ]作離體(in vitro)實驗，由結果顯示：(1)九節木50%酒精粗提取(PR)在血小板懸浮液中，能夠抑制U46619、Thrombin、膠原蛋白、血小板活化因子或花生四烯酸所引起的凝集，其IC50分別為 226.7、343.0、198.2、298.2、70.6 μg / ml。(2)九節木氯仿提取物(PRCHCl3)在血小板懸浮液中，能夠抑制 U46619、Thrombin、膠原蛋白、血小板活化因子或花生四烯酸所引起的凝集，其IC50分別為 91.3、601.7、45.0、169.8、78.8 μ g/ml。(3)九節木正丁醇提取物(PRBuOH)在血小板懸浮液中，能夠抑制 U46619、Thrombin、膠原蛋白、血小板活化因子或花生四烯酸所引起的凝集，其IC50分別為83.6、227.8、80.9、88.6、38.5 μ g /ml。綜合上述結果，九節木確具有活血化瘀之效，而以正丁醇提取物之效價最高，但其有效成分的分析仍在進一步探討。

Psychotria rubra (Lour.) Poir. (P.R.) is one of the plant Rubiaceae. Four specious of the genus Psychotria have been found in Taiwan. Recently, the antithrombotic effect of 50% ethanolic extract of P.R. was evaluated in vitro system. The preliminary result is shown that it inhibit platelet aggregation induced by U46619, thrombin, collagen (Coll), platelet-activating-factor (PAF), and arachidonic acid (AA) , with the inhibitory concentration (IC50) 226.7, 343.0, 198.2, 298.2, and 70.6 μ g /ml, respectively. Then, the 50% ethanolic extract of P.R. was further partitioned with n-hexane, chloroform, and n-butanol, respectively. Again, it was found that chloroform and n-butanol fractions inhibited the U46619-, thrombin-, Coll-, PAF-, and AA-induced platelet aggregation. The IC50 of the former was 91.3, 601.7, 45.0, 169.8 and 78.8 μ g/ml, and the later was 83.6, 277.8, 80.9, 88.6 and 38.5 μ g/ml, respectively.