芍藥藥材加工方法與基準方劑中芍藥？含量變化之探討

Studies of Paeoniflorin Contents in Paeonia Lactifloria from Different Preparations and Prescriptions in Taiwan

本研究以高效液相層析(HPLC)對不同來源芍藥藥材中paeoniflorin與以定量分析，比較其不同加工方式對成分的影響，以及探討中藥基準方中含芍藥基準方劑中paeoniflorin的移行率，以作為藥材良窳選擇與實施中藥基準方後中藥製劑的參考，以助中藥的現代化與科學化。結果發現，蠲痹湯之標準湯劑及浸膏劑之移行率為單味藥材之83.86%及73.69%；血府逐瘀湯為67.69%及66.36%；小青龍湯為91.56%及88.39%；四物湯為91.17%及91.49%；百合固金湯為95.40%及90.72%；桂枝湯為80.22%及83.60%；八珍湯為90.84%及82.50%；獨活寄生湯為81.56%及82.44%；完帶湯為86.42%及77.56%。至於不同加工方式之芍藥藥材所含paeoniflorin之含量實驗部分，確定了杭芍與亳芍於原產地之加工方式不同，杭芍為先削皮後煎煮，亳芍為先煎煮後削皮。

In order to proceed a stable quality for Chinese preparations, the government promulgated GMP projects in conduction of the manufactory of Concentrated Extract Chinese Herbal Medicine. The Department of Health also standardized the Chinese Herbal Preparations for the control of quality. It is important to preserve the bioactive constituents while processing concentration of Chinese Herbal Medicine. In the present study, the turnover rates of paeoniflorin from different preparations and prescriptions were evaluated by HPLC quantities. The turnover rates of paeoniflorin were defined as the percentage yields of this component in different Chinese Medical Prescriptions. The results indicated that the turnover rates of Combination and Extracts in Juan-Bi Tang were 83.８６% ad 73.69%, respectively. The rates was 67.69% and 66.36% in Xue-Fu-Zu-Yu Tang; 91.56% and 88.39% in Xiao-Qing-Long Tang; 91.17% and 91.49% in Shy-Wuh Tang; 95.40% and 90.72% in Bai-He-Gu-Jin Tang; 80.22% and 83.60% in Gui-Zhi Tang; 90.84% and 82.50% in Ba-Zhen Tang; 81.56% and 82.44% in Du-Huo-Ji-Sheng Tang; 86.42% and 77.56% in Wan-Dai Tang. Besides, Hanh-Shao and Buo-Shao were prepared differently in the origin sites. Hanh-Shao was pealed before further agitating. Buo-Shao was agitated before peeling.