Pharmacological active ingredients obtained from Chinese herbs are proved for many years. However, the mystery of combining various herbs become effective in many Chinese herbs combination is unsolved. Furthermore, the mechanism(s) of several most frequently used herbs, so-called Chiense herbal enhancer, contained in over 30-75% of Chinese medicines are still unknown. The present report contains: (1) postulating possible mechanism(s) of Chinese herbal enhancer (2) examining mechanism(s) by study the transportation of drug through membrane, skin in vitro and in vivo. (3) performing clinical study to validate the preclinical findings.