利用高效液相層析法測定粉防己鹼及其在大白鼠之藥物動力學研究

Determination of Tetrandrine with High-Performance Liquid Chromatography and Its Application to Pharmacokinetic Study in Rats

本研究建立套高效液相層析儀的分法，來定量分析大白鼠血漿中粉防己鹼的濃度。液相層析的固定相分離管柱是以耐酸性的逆相C18層析管柱進行，移動相溶媒組成是 acetonitrile-water-diethylamine(20:80:0.1,v/v/v, pH 3.0-3.5，以過氯酸調整 )。當紫外線光譜偵測器波長設在 235nm 時，此高效液相層析儀對大白鼠血漿中粉防己鹼濃度的 偵測極限是 0.03ug/ml。在藥物動力學的研究中，以13mg/kg 的粉防己鹼劑量靜脈投與大 白鼠，由血漿中粉防己鹼濃度與採血時間的關係得知，粉防己鹼在大白鼠體內呈現出二室體 的藥物動力學模式。粉防己鹼在大白鼠體內的排除半衰期及身體總清除率，分別為 42.65 分鐘及 0.36 ml/min/kg。

A high-performance liquid chromatographic method was described for quantitating tetrandrine in rat plasma. The separation was achieved on a liquid chromatography reversed phase 5C18-AR column with a mobile phase of acetonitrile-water-diethylamine (20:80:0.1, v/v/v. pH 3.0-3.5 adjusted with perchloric acid). The detection limit was 0.03μtg/m1 for tetrandrine at a wavelength of 235 nm. The plasma concentration time profile of tetrandrine in rat was suited to a two-compartment open model at a dose of 13mg/kg, intravenously. The elimination half-life and the total body clearance of tetrandrine were 42.65 mm and 0.36 ml/mm/kg in rat.