五味子中Schizandrin及Gomisin A定量及抗肝損傷之研究

Quantitative Analysis of Schizandrin and Gomisin a and Liver Protective Activity of Schisandrae Frutctus

五味子為中醫常用藥材，其成分與藥理作用均被廣泛研究。本研究以水、50%酒精及95%酒精三種不同溶媒分別抽取五味子並以高效液相層析分析，比較得知以95%酒精抽取五味子其中schizandrin及gomisin A含量最高，50%酒精抽取液次之，以水抽取者含量最低。由抗肝損傷藥理試驗顯示95%酒精抽取物亦具有較高的生物活性，水抽出物活性低，可提供五味子萃取及入藥時的參考。此外，急性毒性試驗結果亦發現五味子水、50%酒精及95%酒精三種抽取物對鼷鼠之半數致死劑量(LD50)分別為1.60 g/kg，1.75 g/kg及1.00 g/kg，可見五味子具有一定的毒性，使用時應注意其劑量之控制。

Schisandrae Fructus, the fruit of Schisandra chinensis (Turcz.) Baill is a traditional Chinese medicine. Its constituents and pharmacological activities had been extensively studied. Schisandrae Fructus was extracted by water. 50% alcohol and 95% alcohol respectively and were quantitatively analyzed by high performance liquid chromatography (HPLC). The content of schizandrin and gomisin A in 95% alcohol extracts was highest followed by 50% alcohol extract and water extract. The 95% alcohol extracts also showed higher liver protective activity. The acute toxicity LD□ was 1.0-1.6g/kg (oral; 95% confidence 0.67-2.43g/kg) of Schisandrae Fructus extract.