各種活血化瘀中藥所含三亞麻油酸甘油酯之高效液相層析定量分析法

Determination of Trilinolein in the Vasoactive and Antithrombotic Herbs by HPLC

我們應用一種改良過的高效液相層析法，以聚合逆相碳18的分離管柱(polymeric reversed-phase C18)及移動相為acetonitrile：methanol化氣(1:1, v/v)來沖提出人參等八種中藥之n-hexane抽提物中的三亞麻油酸甘油酯。定量分析出來的三亞麻油酸甘油酯，可在波長205nm偵測到。取三亞麻油酸甘油酯濃度0

An improved high-performance liquid chromatographic method was useded to determinate trilinolein, the active triacylglycerol principles of Ginseng Radix (Renshen), Acanthopanacis Radicis Cortex (Ciwujia), Angelicae Sinensis Radix (Danggui), Ligustici Wallichii Rhizoma (Chuanxiong), Bupleuri Radix (Chaihu), Paeoniae Radix Rubra (Chishao), Paeoniae Radix Alba (Baishao) and Salviae Miltiorrhiza Radix (Danshen). Chromatographic analysis is achieved on isocratic system consisting of a polymeric reversed-phase C18 column with a mobile phase of acetonitrile-methanol (50:50, v/v) to elute the trilinolein. The eluted trilinolein is detected at 205nm. The linear calibration range and detection limit for trilinolein were 0.5-20μg/mL and 0.1μg/mL, respectively. It was found that the trilinolein contained in n-hexane extracts of Ginseng Radix (Renshen), Acanthopanacis Radicis Cortex (Ciwujia), Ligustici Wallichii Rhizoma (Chuanxiong), Angelicae Sinensis Radix (Danggui), Bupleuri Radix (Chaihu), Paeoniae Radix Rubra (Chishao), Paeoniae Radix Alba (Baishao) were 0.274±0.009, 0.131±0.007, 4.135±0.071, 0.530±0.017, 0.168±0.005, 0.101±0.002 and 0.443±0.010mg/g, respectively. However, the trilinolein was not detected in the Salviae Miltiorrhiza Radix (Danshen).