The establishment of a successful cell suspension culture system is a prerequisite for the production of secondary metabolites from medicinal herbs. The purpose of this study was to establish the cell suspension culture from petioles of Angelica dahurica var. formosana and to evaluate the suitable cultural condition for the suspension cell growth. It was found that for developing a rapidly growing and finely dispersed cell suspension culture, the best medium composition was 1/2 MS basal salts, 0.5-4mg/l 2, 4-D, 0.5mg/l kinetin and 3-5% sucrose with medium pH of 6-7. The appropriate light intensity was 200lux with a photoperiod of 16 hr cultured under 25±1℃ condition. The shaking speed was suggested to be 120 rpm. The initial volume affected significantly the growth rate of cells in suspension. The stationary growth phase was reached within 12 days with an initial volume of 5ml packed cells in 25ml medium. However, lower volume (0.5-1.0ml) was suggested for long-term suspension culture as the stationary phase was detained to 22 days after culture. In a typical growth cycle of the suspension cells, the stationary phase was reached around the 16th day, whereas the production of imperatorin, the major medicinal compound produced by the culture, was maximum at the 12th day. The cell suspension of A. dahurica var. formosana established from this study could be used as a pilot model for the production of imperatorin.