| 英文摘要 |
The pharmacological effects of the 50% ethanol extract of Chinese herbal drug Gehua Flores Puerariae, FP) was characterized and evaluated in whole animals and isolated organ preparations. The following results were obtained: 1. The median lethal dose (LD50) and its 95% confidence limits of FP for intraperitoneal injection (i. p.) to mice (ICR, Swiss Hauschka) was 9.4 and 9.0-9.9 g (dry weight of FP) per kg body weight, respectively, 2. FP (2 g/kg, i.p.) reduced the locomotor actitivty but had no effect on the treadmill performance of mice. It also prolonged the duration, but not the onset, of loss of the righting reflex in mice induced with 25% ethanol (20 ml/kg, i.p.). 3. In isolated smooth muscle preparations, FP (0.8-6.4 mg/ml) concentration-dependently relaxed 1μM methacholine-and 50 mM KCI-induced contraction of guinea pig tracheal spirals and canine coronary artery spirals, respectively. FP also inhibited the contractile effects of methacholine (10μM) or histamine (10μM) on guinea pig ileums and acetylcholine (0.55 mM) or KCl(100 mM) on rat tracheal spirals. 4. In rat phrenic nerve-diaphragm preparations, FP (8-24 mg/ml) first enhanced, then depressed and finally abolished the twitch response of the diaphragm to either indirect or direct electrical stimulation. Similar results were observed in the presence of d-tubocurarine (3.3μg/ml) or in the absence of extracellular calcium ion. 5. In rat soleus muscle preparations, the contracture response of the soleus muscle to caffeine (4 mM) was enhanced by FP (16 mg/ml). However, the caffeine's effect at high dose (20 mM) was depressed by FP. The curve of cumulative concentration-contracture tension for caffeine (2-20 mM) was shifted leftward and downward by FP (16 mg/ml). All aforementioned effects of FP on isolated organ preparations were concentration-dependent and reversible, therefore they may be used as the bioassay systems to evaluate the bioactive principles from FP. |
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