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篇名
香茹增加TRAIL 誘發肝癌細胞凋亡與c-FLIP 表現降低有關
並列篇名
Glossogyne Tenuifolia Enhances TRAIL-induced Apoptosis in Hepatoma Involving Down Regulation of c-FLIP
作者 戴宗玄洪哲穎許夏芬方麗雯
中文摘要
香茹 (Glossogyne tenuifolia) 為澎湖特有的草藥,近年的研究顯示香茹具有抗發炎、降低B型肝炎病毒表面抗原表現及抑制癌細胞增殖等功效。腫瘤壞死因子相關的凋亡誘導蛋白 (Tumornecrosis factor-related apoptosis inducing ligand, TRAIL) 會造成腫瘤細胞的凋亡,但對正常細胞並不具有毒性。本研究目的在了解香茹是否輔助TRAIL 誘發肝癌細胞凋亡並探討其作用機轉。研究結果顯示香茹乙酸乙酯層 (GT/EA) 可以有效的增加TRAIL 誘發肝癌細胞株 (Hep 3B) 凋亡。利用西方墨點法檢測,發現GT/EA 會促使裁切型ADP- 核酸聚合酶 (Poly (ADP-ribose) polymerase-1,PARP-1) 蛋白質片段大量增加。而細胞型類FLICE 抑制蛋白 (cellular FLICE-like inhibitory protein,c-FLIP)是死亡受器誘發細胞凋亡過程中主要的抑制性蛋白質,結果顯示Hep 3B在GT/EA作用下,c-FLIP 表現量降低。本研究發現香茹具有提升肝癌細胞對TRAIL 敏感度的能力,與c-FLIP 表現降低相關聯,因此促進TRAIL 誘發Hep 3B 的細胞凋亡。
英文摘要
Glossogyne tenuifolia (Hsiang-Ju) is a unique traditional herb medicine in Penghu Islands. It was reported that the extract exhibited the anti-inflammation, reduction of surface antigen of HBV and proliferation inhibition of cancer cells. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) could trigger apoptosis specific in cancer cells but not normal cells. In this study, we aimed to determine the mechanisms that G. tenuifolia assist TRAIL-induced apoptosis of hepatoma cell. We found that administration of G. tenuifolia ethyl acetae extract (GT/EA) increased TRAIL-induced apoptosis in hepatoma cell line (Hep 3B). With western blotting, GT/EA could enhance poly (ADP-ribose) polymerase-1 (PARP-1) cleavage in the TRAIL-induced cell death pathway. Cellular FLICE (FADD-like IL-1β-converting enzyme)-inhibitory protein (c-FLIP) is a major anti-apoptotic protein in death receptor, including TRAIL, induced apoptosis. Further examination demonstrated that c-FLIP was downregulated in GT/EA-treated Hep 3B cells. This study concludes that G. tenuifolia could increase TRAIL-induced apoptosis in hepatoma involving down regulation of c-FLIP.
起訖頁 1-8
關鍵詞 香茹細胞凋亡腫瘤壞死因子相關的凋亡誘導蛋白細胞型類FLIcE 抑制蛋白Glossogyne tenuifoliaapoptosisTRAILc-FLIP
刊名 長庚科技學刊  
期數 201506 (22期)
出版單位 長庚科技大學
該期刊-下一篇 低升糖指數飲食對於體重過重或肥胖女性體組成及脂質代謝之影響
 

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