乳癌細胞（MCF-7）於低溫冷凍存活率實驗之探討

Investigation of survival rate of breast cancer cell (MCF-7) in the low temperature freezing

對於現今之冷凍治療大多是利用超低溫急速冷凍，使癌細胞凋亡或是壞死；而本實驗研究以非超低溫冷凍方法對乳癌細胞進行冷凍探討，並發現抑制癌細胞之存活率關鍵因素在於結凍，使其開始凋亡(apoptisis)或是壞死(necrosis)以有效抑制乳癌細胞。實驗數據發現，溫度越低，存活率相對降低；冷凍時間延長，則乳癌細胞存活率亦相對降低。以-30°C、10分鐘冷凍實驗，直接量測的存活率與放置三天的存活率相比較，無明顯之恢復情形。由於抑癌藥物具有強烈之副作用，例如：心悸、嘔吐、噁心等，為了降低藥物所帶來之副作用，故進一步將冷凍與抑癌藥物結合以降低用藥劑量，進而達到相同之抗癌效果。冷凍治療對於健康細胞組織會有周圍區域性之凍傷、血管淤塞造成組織壞死之可能性，本研究採用非超低溫冷凍，主要使健康細胞組織之傷害(如凍傷、壞死等)減至最小；以低溫下使乳癌細胞於冷凍手術中有效結凍所造成之影響及冷凍與藥物對癌細胞抑制之相關分析。

The cryosurgery treatment methods mostly use ultra-low temperature to rapidly freeze the treated cells to make the cancer cell apoptosis or necrosis. This experimental study carried on the freezing discussion by the non-ultralow temperature freezing method to the breast cancer cell, and discovered that suppressing the breast cancer survival percentage of the main factor is freezing to make it to start perishing apoptosis or necrosis by suppressing the breast cancer cell effectively. The data pointed out that the temperate is lower, and the survival percentage of breast cancer cell is lower; and furthermore the freezing times are longer, and the survival percentage is also lower. Under the -30 °C, 10 minutes freezing experiment, we compared with the direct and after three days survival percentage of breast cancer cell are not obvious restoration situation. The anticancer treatment medicine (doxorubicin) had intense side effect, such as palpitation, vomit, disgusting and so on. To combine freezing and medicine to reduce freezing time and medication dosage in order to reduce side effect of the medicine and hope to decrease medication dosage, then achieve the same effect of suppressing cancer cell. Using the ultra-low temperature cryotherapy method will damage, frostbite and necrosis the surrounding healthy cells and tissues. It is evident that non-ultra-low temperature freezing method could reduce the injury (i.e. frostbite, necrosis etc.) of the surrounding healthy cells and tissues. This research aims at the breast cancer cell under low temperate made the influence of freezes effectively in cryosurgery ,and the freezing and medicine suppress the cancer cell analysis of correlation.