| 英文摘要 |
Contaminated blood culture affects physicians' bloodstream infection judgment and antibiotics use. The study collected 595 patients who required performing blood culture in the emergency room of a medical center from April 2009 to November 2010. The procedures of iodine disinfection of the skin divided into two groups (30 seconds and 2 minutes), randomly collecting 381 blood culture specimens individually (research samples). According to above classification, we could observe their effects on blood culture contamination rate and compare the correlation of the blood culture contamination rate with different phlebotomists caring for the same patient. The results showed that two groups, whether 30 seconds and 2 minutes, had one set of blood culture specimen contamination (contamination rate, 0.26%). As a result, iodine disinfection on skin period was not the main reason of blood culture contamination (P=1.00). However, 981 blood culture sets from the sample patients (non-research samples) were collected by ER staffs, of which 23 sets were contaminated, and the contamination rate was 2.34%. It indicated that the blood culture contamination rates were different between different phlebotomists (P<0.05). Some factors will affect the accuracy of the bacterial isolation result, including collection timing, quantity of blood culture set, position disinfection and disinfection procedure of the blood culture bottles, thus phlebotomists require skillful technique and sterilization conception to decrease blood culture contamination rate and provide doctor correct bacterial culture results . |
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