應用特徵分析探索有向網絡之拓撲結構

Applying Eigen Analysis to Explore Topological Structures of Directed Networks

目的：於飼料中以富含β-glucan的大麥片以取代部分之可溶性纖維，觀察動物實驗模式評估血清中脂肪、脂蛋白及肝臟的脂蛋白濃度以及是否有脂肪肝的影響。方法：實驗棌用倉鼠（Hamster），分成控制組與實驗組（600g、1200g、3000g劑量之大麥片於飼料中）共四組，每組為雄鼠10隻，實驗基礎飼料為添加0.5％膽固醇的高油脂飼料。實驗為期8週（第0週犧牲一批作為基準，第四週、第八週分別犧牲動物），取其血液、肝臟樣本分析脂質及脂蛋白濃度的差異性。結果：血清樣本分析，控制組與實驗組之總膽固醇濃度（TC）之差異，分別為-12%，-8.7%、-10.7%；三酸甘油酯濃度（TG）之差異，1200g與3000g劑量大麥片組分別為-40.3%、-17.6%。高密度脂蛋白-膽固醇（HDL-C）濃度，控制組與實驗組無差異性，但是低密度脂蛋白-膽固醇（LDL-C），則有顯著差異，分別為-12.3%，-13%、-11.2%。肝臟樣本分析，控制組與實驗組於低密度脂蛋白－膽固醇（LDL-C）有差異性，分別為-40%、-33%、-38.8%。高密度脂蛋白-膽固醇（HDL-C），只有1200g劑量大麥片組為+114.3%，其餘2組則無顯著性差異。結論：由實驗結果顯示，在飼料當中添加不同劑量的大麥片，可以降低高膽固醇血症之倉鼠之血清中總膽固醇（TC）、三酸甘油酯（TG）與低密度脂蛋白-膽固醇（LDL-C）。於肝臟中，高密度脂蛋白-膽固醇（HDL-C）與低密度脂蛋白-膽固醇（LDL-C），則有不同程度的影響。

Purpose: As an animal experimental model created for observation, barley flakes containing high level of β-glucan were used to replace part of the soluble fiber in animal feed to assess its effects on serum lipids and lipoproteins, lipoproteins in liver, and fatty liver. Method: Hamsters were divided into a total of four groups: one control group and three experimental groups (feed containing 600g, 1200g and 3000g of barley flakes, respectively). Ten male hamsters were assigned per group, and the basal feed is the high-fat diet containing 0.5% cholesterol. The experiment was conducted for 8 weeks (a group of hamsters was sacrificed at week 0 as the baseline, and the remaining animals were sacrificed at week 4 and week 8). Blood and liver samples were collected for analysis of differences in the concentrations of lipids and lipoproteins. Result: Serum sample analysis indicated that the differences in total cholesterol (TC) concentration between the control and experimental groups were -12%, -8.7% and -10.7%, respectively; the differences in triglyceride (TG) concentration of the 1200g and 3000g group were -40.3% and -17.6%, respectively. No significant difference was observed in the concentration of high-density lipoprotein-cholesterol (HDL-C) between the control and experimental groups, whereas significant differences in the concentration of low-density lipoprotein-cholesterol (LDL-C) were found between the groups, -12.3%, -13% and -11.2%, respectively. Liver sample analysis showed that the differences in LDL-C level between the control and three experimental groups were -40%, -33% and -38.8%, respectively, and the differences were significant. Moreover, only the 1200g group showed a significant difference in the concentration of HDL-C (+114.3%) and no significant differences were observed in the remaining two groups. Conclusion: According to the results, the addition of different levels of barley flakes to the feed can lower serum TC, TG and HDL-C concentrations in hamsters with hypercholesterolemia. In addition, barley flakes have different effects on HDL-C and LDL-C levels in the liver.